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Ubiquitous urease affects soybean susceptibility to fungi

Wiebke-Strohm, Beatriz ; Pasquali, Giancarlo ; Margis-Pinheiro, Márcia ; Bencke, Marta ; Bücker-Neto, Lauro ; Becker-Ritt, Arlete B. ; Martinelli, Anne H. S. ; Rechenmacher, Ciliana ; Polacco, Joseph C. ; Stolf, Renata ; Marcelino, Francismar C. ; Abdelnoor, Ricardo V. ; Homrich, Milena S. ; Del Ponte, Emerson M. ; Carlini, Celia R. ; De Carvalho, Mayra C. C. G. ; Bodanese-Zanettini, Maria Helena

Plant molecular biology, 2012-05, Vol.79 (1-2), p.75-87 [Periódico revisado por pares]

Dordrecht: Springer Netherlands

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Título:
Ubiquitous urease affects soybean susceptibility to fungi
Autor: Wiebke-Strohm, Beatriz ; Pasquali, Giancarlo ; Margis-Pinheiro, Márcia ; Bencke, Marta ; Bücker-Neto, Lauro ; Becker-Ritt, Arlete B. ; Martinelli, Anne H. S. ; Rechenmacher, Ciliana ; Polacco, Joseph C. ; Stolf, Renata ; Marcelino, Francismar C. ; Abdelnoor, Ricardo V. ; Homrich, Milena S. ; Del Ponte, Emerson M. ; Carlini, Celia R. ; De Carvalho, Mayra C. C. G. ; Bodanese-Zanettini, Maria Helena
Assuntos: Antiviral activity ; Basidiomycota - growth & development ; Biochemistry ; Biological Assay ; Biomedical and Life Sciences ; Disease Resistance - genetics ; DNA, Bacterial - genetics ; Enzymatic activity ; Enzymes ; Fungi ; Gene expression ; Gene Expression Regulation, Plant ; Genetic Vectors - genetics ; Genotypes ; Glycine max - enzymology ; Glycine max - genetics ; Glycine max - microbiology ; Infection ; Leaves ; Life Sciences ; Media (differential) ; Penicillium ; Phakopsora pachyrhizi ; Phomopsis ; Plant biology ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant extracts ; Plant Leaves - microbiology ; Plant Pathology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant resistance ; Plant Sciences ; Plants, Genetically Modified ; Recombination, Genetic - genetics ; Recycling ; Rhizoctonia solani ; Soybeans ; Toxicity ; Transformation, Genetic ; Transgenes - genetics ; Transgenic plants ; Urea ; Urea - metabolism ; Urease ; Urease - metabolism
É parte de: Plant molecular biology, 2012-05, Vol.79 (1-2), p.75-87
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Descrição: The soybean ubiquitous urease (encoded by GmEu 4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu 4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu 4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu 4, the transgenic plants exhibited GmEu 4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani , Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from non-transgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu 4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach.
Editor: Dordrecht: Springer Netherlands
Idioma: Inglês

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Ubiquitous urease affects soybean susceptibility to fungi

Dordrecht: Springer Netherlands

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