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Fine specificity and idiotype diversity of a murine anti-HLA-DQw3 monoclonal antibody elicited with a syngeneic antiidiotypic monoclonal antibody

Shinji, T ; Ono, R ; Ferrone, S

The Journal of immunology (1950), 1990-06, Vol.144 (11), p.4291-4297 [Periódico revisado por pares]

Bethesda, MD: Am Assoc Immnol

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Título:
Fine specificity and idiotype diversity of a murine anti-HLA-DQw3 monoclonal antibody elicited with a syngeneic antiidiotypic monoclonal antibody
Autor: Shinji, T ; Ono, R ; Ferrone, S
Assuntos: Animals ; Antibodies, Anti-Idiotypic - immunology ; Antibodies, Monoclonal - immunology ; Antibody Affinity ; Antibody Specificity ; Antigens ; B-Lymphocytes - immunology ; Biological and medical sciences ; Electrophoresis, Gel, Two-Dimensional ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Histocompatibility antigens (hla, h-2 and other systems) ; HLA-DQ Antigens - immunology ; Humans ; Immunoglobulin Idiotypes - immunology ; Mice ; Mice, Inbred BALB C ; Molecular immunology ; Precipitin Tests
É parte de: The Journal of immunology (1950), 1990-06, Vol.144 (11), p.4291-4297
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A total of 469 hybridomas was generated from a BALB/c mouse immunized with the syngeneic antiidiotypic mAb KO3-34 that recognizes an idiotope within the Ag-combining site of the immunizing syngeneic anti HLA-DQw3 mAb KS13. One of the hybridomas, named S2B154, was shown with a number of serologic and immunochemical assays to mimic the specificity of anti HLA-DQw3 mAb KS13. This result was unexpected, because the antiidiotypic mAb had been classified as gamma because of the lack of detection of anti-HLA-DQw3 antibodies in sera from BALB/c mice immunized with the antiidiotypic mAb KO3-34. The antiidiotypic mAb S2B154, an IgG1, displays a lower affinity constant to HLA-DQw3 Ag-bearing lymphoid cells than mAb KS13, an IgG2b, but a higher one to antiidiotypic mAb KO3-34. Testing with a panel of antiidiotypic mAb elicited with the mAb KS13 showed that both antiidiotypic mAb S2B154 and mAb KS13 express in their Ag-combining site the idiotopes recognized by the antiidiotypic mAb KO3-256, KO3-335, and R1-38. However, the antiidiotypic mAb S2B154 does not react with the mAb R18-9 that recognizes an idiotope outside the Ag-combining site of mAb KS13. The results we have presented question the validity of the classification of antiidiotypic antibodies based on their ability to inhibit the binding of the corresponding antibodies to Ag and on the specificity of antisera elicited with antiidiotypic antibodies. Furthermore, the hybridomas we have developed in this Id cascade provide us the opportunity to analyze the structural basis of idiotypic Ag mimicry and to evaluate the regulatory properties of antiidiotypic antibodies in the immune response to HLA class II Ag.
Editor: Bethesda, MD: Am Assoc Immnol
Idioma: Inglês

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Fine specificity and idiotype diversity of a murine anti-HLA-DQw3 monoclonal antibody elicited with a syngeneic antiidiotypic monoclonal antibody

Shinji, T ; Ono, R ; Ferrone, S

Bethesda, MD: Am Assoc Immnol

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