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High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media

HOLO, H ; NES, I. F

Applied and Environmental Microbiology, 1989-12, Vol.55 (12), p.3119-3123 [Periódico revisado por pares]

Washington, DC: American Society for Microbiology

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Título:
High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media
Autor: HOLO, H ; NES, I. F
Assuntos: Biological and medical sciences ; Biotechnology ; culture media ; Diverse techniques ; electricidad ; electricite ; electricity ; experimentation en laboratoire ; experimentos en laboratorio ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; genetic transformation ; glicina ; glycine ; laboratory experiments ; medio de cultivo ; Methods ; Methods. Procedures. Technologies ; milieu de culture ; Molecular and cellular biology ; streptococcus ; transformacion genetica ; transformation genetique ; Vectors (cloning, transfer, expression). Insertion sequences and transposons
É parte de: Applied and Environmental Microbiology, 1989-12, Vol.55 (12), p.3119-3123
Notas: Q
Q02
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Present address: Laboratory of Microbial Gene Technology, P. O. Box 51, N-1432 Ås-NLH, Norway.
Corresponding author.
Descrição: An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be stored at -85 degrees C without loss of competence. With Lactococcus lactis subsp. cremoris BC101, a transformation frequency of 5.7 X 10(7) transformants per microgram of pIL253 DNA was obtained, which represents 5% of the surviving cells. All the lactococcal strains tested could be transformed by the present method.
Editor: Washington, DC: American Society for Microbiology
Idioma: Inglês

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High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media

HOLO, H ; NES, I. F

Washington, DC: American Society for Microbiology

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