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Endophytic root colonization of gramineous plants by Herbaspirillum frisingense

Rothballer, Michael ; Eckert, Barbara ; Schmid, Michael ; Fekete, Agnes ; Schloter, Michael ; Lehner, Angelika ; Pollmann, Stephan ; Hartmann, Anton

FEMS microbiology ecology, 2008-10, Vol.66 (1), p.85-95 [Peer Reviewed Journal]

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

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Title:
Endophytic root colonization of gramineous plants by Herbaspirillum frisingense
Author: Rothballer, Michael ; Eckert, Barbara ; Schmid, Michael ; Fekete, Agnes ; Schloter, Michael ; Lehner, Angelika ; Pollmann, Stephan ; Hartmann, Anton
Subjects: Acetic acid ; Acyl-Butyrolactones - metabolism ; AHL production ; Amino Acids, Cyclic - metabolism ; Animal, plant and microbial ecology ; Antibodies ; Barley ; Biological and medical sciences ; Colonization ; Confocal microscopy ; Cylinders ; Ecology ; Endophytes ; endophytic colonization ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Genes, Reporter ; Green fluorescent protein ; Green Fluorescent Proteins - genetics ; Herbaspirillum - genetics ; Herbaspirillum - growth & development ; Herbaspirillum - metabolism ; Herbaspirillum frisingense ; Hordeum vulgare ; IAA production ; Immunohistochemistry ; Immunology ; Indoleacetic acid ; Indoleacetic Acids - metabolism ; Inoculation ; Lactones ; Localization ; Microbial ecology ; Microbiology ; Microscopy, Confocal ; Miscanthus ; Miscanthus sinensis ; Nitrogen Fixation ; Plant growth ; Plant Growth Regulators - metabolism ; Plant Roots - cytology ; Plant Roots - microbiology ; Plant tissues ; Poaceae - cytology ; Poaceae - microbiology ; Polyclonal antibodies ; Roots ; Scanning microscopy ; Seedlings ; Seedlings - cytology ; Seedlings - microbiology ; Species Specificity ; Vascular tissue
Is Part Of: FEMS microbiology ecology, 2008-10, Vol.66 (1), p.85-95
Notes: http://dx.doi.org/10.1111/j.1574-6941.2008.00582.x
Editor: Kornelia Smalla
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: Herbaspirillum frisingense is a diazotrophic betaproteobacterium isolated from C4-energy plants, for example Miscanthus sinensis. To demonstrate endophytic colonization unequivocally, immunological labeling techniques using monospecific polyclonal antibodies against two H. frisingense strains and green fluorescent protein (GFP)-fluorescence tagging were applied. The polyclonal antibodies enabled specific in situ identification and very detailed localization of H. frisingense isolates Mb11 and GSF30T within roots of Miscanthusxgiganteus seedlings. Three days after inoculation, cells were found inside root cortex cells and after 7 days they were colonizing the vascular tissue in the central cylinder. GFP-tagged H. frisingense strains could be detected and localized in uncut root material by confocal laser scanning microscopy and were found as endophytes in cortex cells, intercellular spaces and the central cylinder of barley roots. Concerning the production of potential plant effector molecules, H. frisingense strain GSF30T tested positive for the production of indole-3-acetic acid, while Mb11 was shown to produce N-acylhomoserine lactones, and both strains were able to utilize 1-aminocyclopropane-1-carboxylate (ACC), providing an indication of the activity of an ACC-deaminase. These results clearly present H. frisingense as a true plant endophyte and, although initial greenhouse experiments did not lead to clear plant growth stimulation, demonstrate the potential of this species for beneficial effects on the growth of crop plants.
Publisher: Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
Language: English

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Endophytic root colonization of gramineous plants by Herbaspirillum frisingense

Rothballer, Michael ; Eckert, Barbara ; Schmid, Michael ; Fekete, Agnes ; Schloter, Michael ; Lehner, Angelika ; Pollmann, Stephan ; Hartmann, Anton

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

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