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Nitric oxide synthase activity in the abdominal aorta of rats is decreased after 4 weeks of simulated microgravity

Ren, Xin-Ling ; Zhang, Ran ; Zhang, Yu-Yang ; Liu, Huan ; Yu, Jin-Wen ; Cai, Yue ; Wang, Zhong-Chao ; Purdy, Ralph E ; Ma, Jin

Clinical and experimental pharmacology & physiology, 2011-10, Vol.38 (10), p.683-687 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Nitric oxide synthase activity in the abdominal aorta of rats is decreased after 4 weeks of simulated microgravity
Autor: Ren, Xin-Ling ; Zhang, Ran ; Zhang, Yu-Yang ; Liu, Huan ; Yu, Jin-Wen ; Cai, Yue ; Wang, Zhong-Chao ; Purdy, Ralph E ; Ma, Jin
Assuntos: abdominal aorta ; Acetylcholine - pharmacology ; Animals ; Aorta, Abdominal - drug effects ; Aorta, Abdominal - enzymology ; Aorta, Abdominal - metabolism ; Aorta, Abdominal - physiology ; Arginine - pharmacology ; Cyclic GMP - analogs & derivatives ; Cyclic GMP - pharmacology ; Male ; Nitrates - metabolism ; nitric oxide synthase ; Nitric Oxide Synthase - metabolism ; Nitric Oxide Synthase Type II - metabolism ; Nitric Oxide Synthase Type III - metabolism ; Nitrites - metabolism ; Nitroprusside - pharmacology ; rat ; Rats ; Rats, Sprague-Dawley ; simulated microgravity ; Time Factors ; vascular reactivity ; Vasodilation - drug effects ; Vasodilation - physiology ; Weightlessness Simulation - methods ; Weightlessness Simulation - statistics & numerical data
É parte de: Clinical and experimental pharmacology & physiology, 2011-10, Vol.38 (10), p.683-687
Notas: ArticleID:CEP5565
istex:9950D3D482994DCF3A5FB9BA4B4A3F2B312BE72F
ark:/67375/WNG-RVK81V6K-5
These authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Summary 1. The aim of the present study was to investigate the effects of simulated microgravity on the arterial dilatory responsiveness and l‐arginine (l‐Arg)–nitric oxide (NO)–cGMP pathway in the abdominal aorta of rats. 2. Twenty healthy male Sprague‐Dawley were randomly divided into control and simulated microgravity groups. Rats in the simulated microgravity group were subjected to hindlimb unweighting (HU). After 4 weeks, arterial dilatory responsiveness was examined in vitro in isolated abdominal aortic rings. Western blotting was used to measure endothelial (e) and inducible (i) NO synthase (NOS) protein content. Total concentrations of nitrate and nitrite (NOx), the stable metabolites of NO, were determined by the chemiluminescence method. Nitric oxide synthase activity in the abdominal aorta was determined through the conversion of [3H]‐l‐Arg to [3H]‐l‐citrulline. 3. The data showed that the dilatory responses of the arterial rings to l‐Arg and acetylcholine decreased in rats exposed to simulated microgravity, but the dilatory responses to sodium nitroprusside and 8‐bromo‐cGMP were similar in both simulated microgravity and control rats. The expression of eNOS and iNOS did not differ significantly between the two groups. The NOx concentration in the abdominal aorta of HU rats was significantly less than that in control rats. Nitric oxide synthase activity in the aorta decreased after 4 weeks of HU. 4. The data indicate that endothelium‐dependent vasorelaxation in the abdominal aorta decreased due to 4 weeks of simulated microgravity in rats and that this impaired dilatory responsiveness may result from decreased NOS activity.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Nitric oxide synthase activity in the abdominal aorta of rats is decreased after 4 weeks of simulated microgravity

Ren, Xin-Ling ; Zhang, Ran ; Zhang, Yu-Yang ; Liu, Huan ; Yu, Jin-Wen ; Cai, Yue ; Wang, Zhong-Chao ; Purdy, Ralph E ; Ma, Jin

Oxford, UK: Blackwell Publishing Ltd

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