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A CRE1- regulated cluster is responsible for light dependent production of dihydrotrichotetronin in Trichoderma reesei

Monroy, Alberto Alonso ; Stappler, Eva ; Schuster, Andre ; Sulyok, Michael ; Schmoll, Monika Nowrousian, Minou

PloS one, 2017-08, Vol.12 (8), p.e0182530-e0182530 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A CRE1- regulated cluster is responsible for light dependent production of dihydrotrichotetronin in Trichoderma reesei
Autor: Monroy, Alberto Alonso ; Stappler, Eva ; Schuster, Andre ; Sulyok, Michael ; Schmoll, Monika
Nowrousian, Minou
Assuntos: Antagonism ; Antibiotics ; Biodegradation ; Biology and Life Sciences ; Biosynthesis ; Carbon ; Cellulase ; Cellulose ; Clusters ; Darkness ; Degradation ; Earth rotation ; Enzymes ; Feedback ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungi ; Gene expression ; Gene Expression Regulation, Fungal - radiation effects ; Gene regulation ; Genes ; Genetic aspects ; Genomes ; Genomics ; Glycoside hydrolase ; Hydrolase ; Light ; Metabolism ; Microbial metabolism ; Monooxygenase ; Negative feedback ; Night ; Photoreceptors ; Physical Sciences ; Physiological aspects ; Positive feedback ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Trichoderma - genetics ; Trichoderma - metabolism ; Trichoderma - radiation effects ; Trichoderma reesei ; VOCs ; Volatile organic compounds
É parte de: PloS one, 2017-08, Vol.12 (8), p.e0182530-e0182530
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: A patent application has been filed which also includes the topic of this study.
Descrição: Changing light conditions, caused by the rotation of earth resulting in day and night or growth on the surface or within a substrate, result in considerably altered physiological processes in fungi. For the biotechnological workhorse Trichoderma reesei, regulation of glycoside hydrolase gene expression, especially cellulase expression was shown to be a target of light dependent gene regulation. Analysis of regulatory targets of the carbon catabolite repressor CRE1 under cellulase inducing conditions revealed a secondary metabolite cluster to be differentially regulated in light and darkness and by photoreceptors. We found that this cluster is involved in production of trichodimerol and that the two polyketide synthases of the cluster are essential for biosynthesis of dihydrotrichotetronine (syn. bislongiquinolide or bisorbibutenolide). Additionally, an indirect influence on production of the peptaibol antibiotic paracelsin was observed. The two polyketide synthetase genes as well as the monooxygenase gene of the cluster were found to be connected at the level of transcription in a positive feedback cycle in darkness, but negative feedback in light, indicating a cellular sensing and response mechanism for the products of these enzymes. The transcription factor TR_102497/YPR2 residing within the cluster regulates the cluster genes in a light dependent manner. Additionally, an interrelationship of this cluster with regulation of cellulase gene expression was detected. Hence the regulatory connection between primary and secondary metabolism appears more widespread than previously assumed, indicating a sophisticated distribution of resources either to degradation of substrate (feed) or to antagonism of competitors (fight), which is influenced by light.
Editor: United States: Public Library of Science
Idioma: Inglês

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A CRE1- regulated cluster is responsible for light dependent production of dihydrotrichotetronin in Trichoderma reesei

Monroy, Alberto Alonso ; Stappler, Eva ; Schuster, Andre ; Sulyok, Michael ; Schmoll, Monika Nowrousian, Minou

United States: Public Library of Science

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