Idioma:

DNA methylation pattern of apoptosis‐related genes in ameloblastoma

Costa, SFS ; Pereira, NB ; Pereira, KMA ; Campos, K ; Castro, WH ; Diniz, MG ; Gomes, CC ; Gomez, RS

Oral diseases, 2017-09, Vol.23 (6), p.779-783 [Periódico revisado por pares]

Denmark

Texto completo disponível

Citações Citado por

Enviar para

Título:
DNA methylation pattern of apoptosis‐related genes in ameloblastoma
Autor: Costa, SFS ; Pereira, NB ; Pereira, KMA ; Campos, K ; Castro, WH ; Diniz, MG ; Gomes, CC ; Gomez, RS
Assuntos: Adult ; ameloblastoma ; Ameloblastoma - genetics ; apoptosis ; Apoptosis - genetics ; Bcl-2-Like Protein 11 - genetics ; Bcl‐2‐like protein 11 ; Case-Control Studies ; Dentistry ; DNA Methylation ; Female ; Gene Expression ; Humans ; Jaw Neoplasms - genetics ; Male ; Middle Aged ; Promoter Regions, Genetic ; Receptors, Tumor Necrosis Factor, Member 25 - genetics ; TNFRSF25 ; Young Adult
É parte de: Oral diseases, 2017-09, Vol.23 (6), p.779-783
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Objectives DNA methylation is an important mechanism of gene control expression, and it has been poorly addressed in odontogenic tumours. On this basis, we aimed to assess the methylation pattern of 22 apoptosis‐related genes in solid ameloblastomas. Materials and Methods Ameloblastoma fresh samples (n = 10) and dental follicles (n = 8) were included in the study. The percentage fraction of methylated and unmethylated DNA promoter of 22 apoptosis‐related genes was determined using enzymatic restriction digestion and quantitative real‐time PCR (qPCR) array. The relative expressions of the genes that showed the most discrepant methylation profile between tumours and controls were analysed by reverse‐transcription quantitative PCR (RT‐qPCR). Results Lower methylation percentages of TNFRSF25 (47.2%) and BCL2L11 (33.2%) were observed in ameloblastomas compared with dental follicles (79.3% and 59.5%, respectively). The RT‐qPCR analysis showed increased expression of BCL2L11 in ameloblastomas compared with dental follicles, in agreement with the methylation analysis results, while there was no difference between the expression levels of TNFRSF25 between both groups. Conclusions On the basis of our results, the transcription of the apoptosis‐related gene BCL2L11 is possibly regulated by promoter DNA methylation in ameloblastoma. The biological significance of this finding in ameloblastoma pathobiology remains to be clarified.
Editor: Denmark
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 9 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

DNA methylation pattern of apoptosis‐related genes in ameloblastoma

Costa, SFS ; Pereira, NB ; Pereira, KMA ; Campos, K ; Castro, WH ; Diniz, MG ; Gomes, CC ; Gomez, RS

Denmark

Buscando em bases de dados remotas. Favor aguardar.