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Distribution of Aquaporin 4 in rodent spinal cord: Relationship with astrocyte markers and chondroitin sulfate proteoglycans

Vitellaro-Zuccarello, Laura ; Mazzetti, Samanta ; Bosisio, Paola ; Monti, Claudia ; De Biasi, Silvia

Glia, 2005-08, Vol.51 (2), p.148-159 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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Título:
Distribution of Aquaporin 4 in rodent spinal cord: Relationship with astrocyte markers and chondroitin sulfate proteoglycans
Autor: Vitellaro-Zuccarello, Laura ; Mazzetti, Samanta ; Bosisio, Paola ; Monti, Claudia ; De Biasi, Silvia
Assuntos: Animals ; Animals, Newborn ; Antigens, Differentiation - biosynthesis ; Aquaporin 4 ; Aquaporins - metabolism ; astrocytes ; Astrocytes - metabolism ; Astrocytes - ultrastructure ; Biological and medical sciences ; Capillaries - physiology ; Capillaries - ultrastructure ; Chondroitin Sulfate Proteoglycans - metabolism ; Excitatory Amino Acid Transporter 2 - metabolism ; extracellular matrix ; Extracellular Matrix - metabolism ; Fundamental and applied biological sciences. Psychology ; GFAP ; Glial Fibrillary Acidic Protein - metabolism ; GLT-1 ; Immunohistochemistry ; Isolated neuron and nerve. Neuroglia ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Confocal ; Microscopy, Electron, Transmission ; Neuropil - metabolism ; Neuropil - ultrastructure ; Presynaptic Terminals - metabolism ; Presynaptic Terminals - ultrastructure ; Rats ; Rats, Sprague-Dawley ; Spinal Cord - metabolism ; Spinal Cord - ultrastructure ; Synaptic Transmission - physiology ; Vertebrates: nervous system and sense organs ; water homeostasis ; Water-Electrolyte Balance - physiology ; WFA
É parte de: Glia, 2005-08, Vol.51 (2), p.148-159
Notas: ArticleID:GLIA20196
University of Milan (FIRST)
istex:C0E40B7E82C0BB8CF651F4614ADF510F63691D25
ark:/67375/WNG-K0459S02-8
Ministero dell'Istruzione dell'Università e della Ricerca (MIUR; Rome, Italy) - No. COFIN 03
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
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Descrição: Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system, as demonstrated by its perturbations in pathological conditions. Aquaporin 4 (AQP4) is the predominant water channel in brain and spinal cord, where it is present mainly on astrocytic endfeet contacting vessels. A role in water homeostasis control has been proposed also for the extracellular matrix, that in brain consists mainly of chondroitin sulfate proteoglycans (CSPGs). Using cytochemical and immunocytochemical techniques, we investigated their distribution in rodent spinal cord, to better understand the role of these two classes of molecules. The results show that in spinal gray matter AQP4 labeling is intense in all perivascular profiles and (1) displays a marked dorsoventral gradient in the neuropil; and (2) coexists extensively with glial glutamate transporter‐1 (GLT‐1) but scarcely with glial fibrillary acidic protein (GFAP). In white matter the overlap between AQP4, GLT‐1, and GFAP is almost complete. Ultrastructural examination shows that AQP4‐labeled astrocytic processes surround blood vessels, neuronal perikarya and processes, and both asymmetric and symmetric synapses, indicating that the protein may be involved in the regulation of water fluxes around both inhibitory and excitatory synapses. CSPGs, visualized by labeling with Wisteria floribunda agglutinin, show a distribution complementary to that of AQP4, being absent or weekly expressed in AQP4‐enriched areas. These findings suggest that different mechanisms may contribute to the regulation of water homeostasis in different spinal cord regions. © 2005 Wiley‐Liss, Inc.
Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Idioma: Inglês

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Distribution of Aquaporin 4 in rodent spinal cord: Relationship with astrocyte markers and chondroitin sulfate proteoglycans

Vitellaro-Zuccarello, Laura ; Mazzetti, Samanta ; Bosisio, Paola ; Monti, Claudia ; De Biasi, Silvia

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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