Idioma:

A critical role for Pol II CTD phosphorylation in heterochromatic gene activation

Kainth, Amoldeep S. ; Zhang, Hesheng ; Gross, David S.

Gene, 2024-08, Vol.918, p.148473-148473, Article 148473 [Periódico revisado por pares]

Netherlands: Elsevier B.V

Texto completo disponível

Citações Citado por

Enviar para

Título:
A critical role for Pol II CTD phosphorylation in heterochromatic gene activation
Autor: Kainth, Amoldeep S. ; Zhang, Hesheng ; Gross, David S.
Assuntos: Bur1 ; ChIP ; CTD code ; Ctk1 ; Euchromatin ; Fluorescence Microscopy ; Gene Activation ; Gene Expression Regulation, Fungal ; Heat Shock ; Heterochromatin ; Heterochromatin - genetics ; Heterochromatin - metabolism ; Histone Code ; Histones - metabolism ; Nucleosomes ; Phosphorylation ; RNA Polymerase II ; RNA Polymerase II - genetics ; RNA Polymerase II - metabolism ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Serine - metabolism ; Sir2/3/4-mediated Silent Chromatin ; Transcription ; Transcriptional Activation ; Yeast
É parte de: Gene, 2024-08, Vol.918, p.148473-148473, Article 148473
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: •Heterochromatic gene expression in the model eukaryote Saccharomyces cerevisiae occurs in the absence of chromatin modifications typically associated with transcriptional activation.•Activation of a model SIR-regulated transgene occurs within a majority of cells in the population.•YFR057w, a telomere-linked heterochromatic gene and strongly induced following exposure to cycloheximide, is transcribed by Pol II molecules enriched in phosphorylated Ser2 residues.•Activation of YFR057w is critically dependent on the Pol II CTD Ser2 kinases Ctk1 and Bur1. How gene activation works in heterochromatin, and how the mechanism might differ from the one used in euchromatin, has been largely unexplored. Previous work has shown that in SIR-regulated heterochromatin of Saccharomyces cerevisiae, gene activation occurs in the absence of covalent histone modifications and other alterations of chromatin commonly associated with transcription.Here we demonstrate that such activation occurs in a substantial fraction of cells, consistent with frequent transcriptional bursting, and this raises the possibility that an alternative activation pathway might be used. We address one such possibility, Pol II CTD phosphorylation, and explore this idea using a natural telomere-linked gene, YFR057w, as a model. Unlike covalent histone modifications, we find that Ser2, Ser5 and Ser7 CTD phosphorylated Pol II is prevalent at the drug-induced heterochromatic gene. Particularly enriched relative to the euchromatic state is Ser2 phosphorylation. Consistent with a functional role for Ser2P, YFR057w is negligibly activated in cells deficient in the Ser2 CTD kinases Ctk1 and Bur1 even though the gene is strongly stimulated when it is placed in a euchromatic context. Collectively, our results are consistent with a critical role for Ser2 CTD phosphorylation in driving Pol II recruitment and transcription of a natural heterochromatic gene – an activity that may supplant the need for histone epigenetic modifications.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 3 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

A critical role for Pol II CTD phosphorylation in heterochromatic gene activation

Kainth, Amoldeep S. ; Zhang, Hesheng ; Gross, David S.

Netherlands: Elsevier B.V

Buscando em bases de dados remotas. Favor aguardar.