skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Single‐cell RNA sequencing technologies and applications: A brief overview

Jovic, Dragomirka ; Liang, Xue ; Zeng, Hua ; Lin, Lin ; Xu, Fengping ; Luo, Yonglun

Clinical and translational medicine, 2022-03, Vol.12 (3), p.e694-n/a [Periódico revisado por pares]

United States: John Wiley & Sons, Inc

Texto completo disponível

Citações Citado por
  • Título:
    Single‐cell RNA sequencing technologies and applications: A brief overview
  • Autor: Jovic, Dragomirka ; Liang, Xue ; Zeng, Hua ; Lin, Lin ; Xu, Fengping ; Luo, Yonglun
  • Assuntos: 16th century ; Animals ; big data ; Clinical medicine ; Gene expression ; Gene Expression Profiling - methods ; Genomics ; Methods ; precision medicine ; regenerative medicine ; Review ; Reviews ; RNA sequencing ; Sequence Analysis, RNA - methods ; single cell ; Single-Cell Analysis - methods ; Technology ; Whole Exome Sequencing
  • É parte de: Clinical and translational medicine, 2022-03, Vol.12 (3), p.e694-n/a
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-3
    content type line 23
    ObjectType-Review-1
  • Descrição: Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as well as revealing the composition of different cell types and functions within highly organized tissues/organs/organisms. Since its first discovery in 2009, studies based on scRNA‐seq provide massive information across different fields making exciting new discoveries in better understanding the composition and interaction of cells within humans, model animals and plants. In this review, we provide a concise overview about the scRNA‐seq technology, experimental and computational procedures for transforming the biological and molecular processes into computational and statistical data. We also provide an explanation of the key technological steps in implementing the technology. We highlight a few examples on how scRNA‐seq can provide unique information for better understanding health and diseases. One important application of the scRNA‐seq technology is to build a better and high‐resolution catalogue of cells in all living organism, commonly known as atlas, which is key resource to better understand and provide a solution in treating diseases. While great promises have been demonstrated with the technology in all areas, we further highlight a few remaining challenges to be overcome and its great potentials in transforming current protocols in disease diagnosis and treatment. This review provides a concise summary of the single‐cell RNA sequencing technologies. Overview and guidelines for planning experimental procedures are presented. Bioinformatics tools for scRNA‐seq data analysis are thoroughly discussed. Applications and further development of scRNA‐seq technology are highlighted.
  • Editor: United States: John Wiley & Sons, Inc
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.