skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Handbook of detection of enzymes on electrophoretic gels

Gennady P. Manchenko

Boca Raton, Fla. CRC Press c1994

Localização: CQ - Conjunto das Químicas    (R 574.1925 M268h ) e outros locais(Acessar)

  • Título:
    Handbook of detection of enzymes on electrophoretic gels
  • Autor: Gennady P. Manchenko
  • Assuntos: Enzymes -- Purification -- Handbooks, manuals, etc; Gel electrophoresis -- Handbooks, manuals, etc; ENZIMAS -- ISOLAMENTO E PURIFICAÇÃO -- ANÁLISE -- MÉTODOS ANALÍTICOS; ELETROFORESE EM GEL -- ANÁLISE -- MÉTODOS ANALÍTICOS; ELETROFORESE -- ANÁLISE -- MÉTODOS ANALÍTICOS; Enzymes -- isolation & purification -- laboratory manuals; Electrophoresis, Polyacrylamide Gel -- laboratory manuals; Enzymes
  • Notas: Includes bibliographical references
  • Descrição: General principles of enzyme detection on electrophoretic gels -- Chromogenic reactions -- Products reducing tetrazolium salts -- Products capable of coupling with diazonium salts -- Products that cause a pH change -- Orthophos phate -- Pyrophosphate -- Hydrogen peroxide -- Carbonate ions -- Colored products -- Products bearing reduced thiol groups -- Products that influence the starch-iodine reaction -- Products of polymerizing enzymes -- Products of depolymerizing enzymes -- Fluorogenic reactions -- NADH and NADPH -- 4-methylumbelliferone Products that form luminescent lanthanide chelates -- Miscellany -- Autoradiography -- Bioautography -- Two-dimensional gel spectroscopy -- Immunoblotting -- Immobilization matrices -- Transfer of proteins -- Specific antibodie s and labeled anti-antibodies -- Detection of transferred proteins on blots -- Methods of detection of specific enzymes -- Structure of enzyme sheets -- General considerations, comments and recommendations -- Choice of support medium for enzyme electrophoresis and detection -- Cellulose acetate gel -- Polyacrylamide gel Starch gel -- Strategies of gel staining -- Choice of zymogram method -- Preparation of staining solution -- Modes of application of staining solutions -- Modes of enhancement of staining intensity of enzyme activity bands -- S pecificity of zymogram methods and some related problems -- Recording and preservation of zymograms -- Resource-saving strategies --
    Simultaneous detection of several enzymes on the same gel -- Successive detection of different enzymes on the same gel -- Reusing of staining solutions -- Use of several origins on one gel Multiple replication of electrophoretic gels by electroblotting -- Use of semipreparative gels for production of preparations of linking enzymes -- Troubleshooting -- Safety regulations -- Enzyme sheets -- Minimal medium for Es cherichia coli -- Citrate medium for Pediococcus cerevisiae -- Alphabetical list of enzymes
  • Editor: Boca Raton, Fla. CRC Press
  • Data de criação/publicação: c1994
  • Formato: 341 p ill. 29 cm..
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.