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Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils

Kobayashi, Scott D. ; Porter, Adeline R. ; Dorward, David W. ; Brinkworth, Amanda J. ; Chen, Liang ; Kreiswirth, Barry N. ; DeLeo, Frank R.

The Journal of infectious diseases, 2016-05, Vol.213 (10), p.1615-1622 [Peer Reviewed Journal]

United States: Oxford University Press

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Title:
Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils
Author: Kobayashi, Scott D. ; Porter, Adeline R. ; Dorward, David W. ; Brinkworth, Amanda J. ; Chen, Liang ; Kreiswirth, Barry N. ; DeLeo, Frank R.
Subjects: Animals ; BACTERIA ; Bacterial Typing Techniques ; Carbapenems - pharmacology ; Drug Resistance, Bacterial ; Female ; Humans ; Klebsiella Infections - microbiology ; Klebsiella Infections - therapy ; Klebsiella pneumoniae ; Klebsiella pneumoniae - drug effects ; Klebsiella pneumoniae - genetics ; Klebsiella pneumoniae - immunology ; Major and Brief Reports ; Multilocus Sequence Typing ; Neutrophils - immunology ; Neutrophils - metabolism ; Neutrophils - microbiology ; Phagocytosis ; Rabbits ; Reactive Oxygen Species - metabolism
Is Part Of: The Journal of infectious diseases, 2016-05, Vol.213 (10), p.1615-1622
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Present affiliation: School of Molecular Biosciences, Washington State University, Pullman.
Description: Carbapenem-resistant Klebsiella pneumoniae strains classified as multilocus sequence type 258 (ST258) are among the most widespread multidrug-resistant hospital-acquired pathogens. Treatment of infections caused by these organisms is difficult, and mortality is high. The basis for the success of ST258, outside of antibiotic resistance, remains incompletely determined. Here we tested the hypothesis that ST258 K. pneumoniae has enhanced capacity to circumvent killing by human neutrophils, the primary cellular defense against bacterial infections. There was limited binding and uptake of ST258 by human neutrophils, and correspondingly, there was limited killing of bacteria. On the other hand, transmission electron microscopy revealed that any ingested organisms were degraded readily within neutrophil phagosomes, thus indicating that survival in the neutrophil assays is due to limited phagocytosis, rather than to microbicide resistance after uptake. Our findings suggest that enhancing neutrophil phagocytosis is a potential therapeutic approach for treatment of infection caused by carbapenem-resistant ST258 K. pneumoniae.
Publisher: United States: Oxford University Press
Language: English

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Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils

Kobayashi, Scott D. ; Porter, Adeline R. ; Dorward, David W. ; Brinkworth, Amanda J. ; Chen, Liang ; Kreiswirth, Barry N. ; DeLeo, Frank R.

United States: Oxford University Press

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