Idioma:

Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3'-UTRs

Kubo, Tomohiro ; Wada, Tadashi ; Yamaguchi, Yuki ; Shimizu, Akira ; Handa, Hiroshi

Nucleic acids research, 2006-12, Vol.34 (21), p.6264-6271 [Periódico revisado por pares]

England: Oxford Publishing Limited (England)

Texto completo disponível

Citações Citado por

Enviar para

Título:
Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3'-UTRs
Autor: Kubo, Tomohiro ; Wada, Tadashi ; Yamaguchi, Yuki ; Shimizu, Akira ; Handa, Hiroshi
Assuntos: 3' Untranslated Regions - chemistry ; cultured cells ; DNA Helicases - genetics ; DNA Repair Enzymes - genetics ; gene expression ; genes ; HeLa Cells ; Humans ; mammals ; messenger RNA ; mRNA Cleavage and Polyadenylation Factors - antagonists & inhibitors ; mRNA Cleavage and Polyadenylation Factors - genetics ; mRNA Cleavage and Polyadenylation Factors - physiology ; Northern blotting ; Poly A - analysis ; Poly-ADP-Ribose Binding Proteins ; Polyadenylation ; Protein Subunits - antagonists & inhibitors ; Protein Subunits - genetics ; Protein Subunits - physiology ; RNA ; RNA Interference ; RNA Splicing ; RNA, Messenger - metabolism ; RNA-binding proteins ; Syndecan-2 - genetics ; Tetrahydrofolate Dehydrogenase - genetics ; Tissue Inhibitor of Metalloproteinase-2 - genetics
É parte de: Nucleic acids research, 2006-12, Vol.34 (21), p.6264-6271
Notas: http://www.nar.oupjournals.org/
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Correspondence may also be addressed to Tadashi Wada. Email: tawada@bio.titech.ac.jp
Descrição: Alternative polyadenylation leads to mRNAs with variable 3' ends. Since a 3'-untranslated region (3'-UTR) often contains cis elements that impact stability or localization of mRNA or translation, selection of poly(A) sites in a 3'-UTR is regulated in mammalian cells. However, the molecular basis for alternative poly(A) site selection within a 3'-UTR has been unclear. Here we show involvement of cleavage factor Im (CFIm) in poly(A) site selection within a 3'-UTR. CFIm is a heterodimeric 3' end-processing complex, which functions to assemble other processing factors on pre-mRNA in vitro. We knocked down 25 kDa subunit of CFIm (CFIm25) in HeLa cells and analyzed alternative poly(A) site selection of TIMP-2, syndecan2, ERCC6 and DHFR genes by northern blotting. We observed changes in the distribution of mRNAs in CFIm25 depleted cells, suggesting a role for CFIm in alternative poly(A) site selection. Furthermore, tissue specific analysis demonstrated that the CFIm25 gene gave rise to 1.1, 2.0 and 4.6 kb mRNAs. The 4.6 kb mRNA was ubiquitously expressed, while the 1.1 and 2.0 kb mRNAs were expressed in a tissue specific manner. We found three likely poly(A) sites in the CFIm25 3'-UTR, suggesting alternative polyadenylation. Our results indicate that alternative poly(A) site selection is a well-regulated process in vivo.
Editor: England: Oxford Publishing Limited (England)
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Resultado 1 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3'-UTRs

Kubo, Tomohiro ; Wada, Tadashi ; Yamaguchi, Yuki ; Shimizu, Akira ; Handa, Hiroshi

England: Oxford Publishing Limited (England)

Buscando em bases de dados remotas. Favor aguardar.