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Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor

Pilewski, Kelsey A. ; Wall, Steven ; Richardson, Simone I. ; Manamela, Nelia P. ; Clark, Kaitlyn ; Hermanus, Tandile ; Binshtein, Elad ; Venkat, Rohit ; Sautto, Giuseppe A. ; Kramer, Kevin J. ; Shiakolas, Andrea R. ; Setliff, Ian ; Salas, Jordan ; Mapengo, Rutendo E. ; Suryadevara, Naveen ; Brannon, John R. ; Beebout, Connor J. ; Parks, Rob ; Raju, Nagarajan ; Frumento, Nicole ; Walker, Lauren M. ; Fechter, Emilee Friedman ; Qin, Juliana S. ; Murji, Amyn A. ; Janowska, Katarzyna ; Thakur, Bhishem ; Lindenberger, Jared ; May, Aaron J. ; Huang, Xiao ; Sammour, Salam ; Acharya, Priyamvada ; Carnahan, Robert H. ; Ross, Ted M. ; Haynes, Barton F. ; Hadjifrangiskou, Maria ; Crowe, James E. ; Bailey, Justin R. ; Kalams, Spyros ; Morris, Lynn ; Georgiev, Ivelin S.

Cell reports (Cambridge), 2023-02, Vol.42 (2), p.112044-112044, Article 112044 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor
Autor: Pilewski, Kelsey A. ; Wall, Steven ; Richardson, Simone I. ; Manamela, Nelia P. ; Clark, Kaitlyn ; Hermanus, Tandile ; Binshtein, Elad ; Venkat, Rohit ; Sautto, Giuseppe A. ; Kramer, Kevin J. ; Shiakolas, Andrea R. ; Setliff, Ian ; Salas, Jordan ; Mapengo, Rutendo E. ; Suryadevara, Naveen ; Brannon, John R. ; Beebout, Connor J. ; Parks, Rob ; Raju, Nagarajan ; Frumento, Nicole ; Walker, Lauren M. ; Fechter, Emilee Friedman ; Qin, Juliana S. ; Murji, Amyn A. ; Janowska, Katarzyna ; Thakur, Bhishem ; Lindenberger, Jared ; May, Aaron J. ; Huang, Xiao ; Sammour, Salam ; Acharya, Priyamvada ; Carnahan, Robert H. ; Ross, Ted M. ; Haynes, Barton F. ; Hadjifrangiskou, Maria ; Crowe, James E. ; Bailey, Justin R. ; Kalams, Spyros ; Morris, Lynn ; Georgiev, Ivelin S.
Assuntos: Antibodies, Neutralizing ; antibody ; antigen cross-reactivity ; antiviral antibodies ; broadly neutralizing ; Coinfection ; COVID-19 ; CP: Immunology ; Hepacivirus ; Hepatitis C ; HIV Antibodies ; HIV Infections ; HIV-1 ; HIV1/HCV co-infection ; Humans ; LIBRA-seq ; SARS-CoV-2 ; somatic hypermutation ; vaccine
É parte de: Cell reports (Cambridge), 2023-02, Vol.42 (2), p.112044-112044, Article 112044
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
AUTHOR CONTRIBUTIONS
Conceptualization and methodology, K.A.P. and I.S.G.; investigation, K.A.P., S.W., S.I.R., N.P.M., K.C., T.H., E.B., R.V., G.A.S., K.J.K., A.R.S., I.S., J.S., R.E.M., N.S., J.R.B., C.J.B., R.P., N.R., N.F., L.M.W., E.F.F., J.S.Q., A.A.M., K.J., B.T., J.L., A.M., X.H., and S.S.; software, K.A.P., E.B., R.V., I.S., N.R., and I.S.G.; writing – original draft, K.A.P. and I.S.G.; writing – review & editing, all authors; funding acquisition, K.A.P., P.A., M.H., J.E.C., and I.S.G.; resources, S.K.; supervision, K.A.P., S.I.R., P.A., R.H.C., T.M.R., B.F.H., M.H., J.E.C., J.R.B., L.M., and I.S.G.
Descrição: Despite prolific efforts to characterize the antibody response to human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) mono-infections, the response to chronic co-infection with these two ever-evolving viruses is poorly understood. Here, we investigate the antibody repertoire of a chronically HIV-1/HCV co-infected individual using linking B cell receptor to antigen specificity through sequencing (LIBRA-seq). We identify five HIV-1/HCV cross-reactive antibodies demonstrating binding and functional cross-reactivity between HIV-1 and HCV envelope glycoproteins. All five antibodies show exceptional HCV neutralization breadth and effector functions against both HIV-1 and HCV. One antibody, mAb688, also cross-reacts with influenza and coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We examine the development of these antibodies using next-generation sequencing analysis and lineage tracing and find that somatic hypermutation established and enhanced this reactivity. These antibodies provide a potential future direction for therapeutic and vaccine development against current and emerging infectious diseases. More broadly, chronic co-infection represents a complex immunological challenge that can provide insights into the fundamental rules that underly antibody-antigen specificity. [Display omitted] •LIBRA-seq is applied to characterize antibody responses to HIV-1/HCV co-infection•A diverse set of functional HIV-1/HCV cross-reactive antibodies are identified•A glycan-directed antibody exhibits exceptionally broad anti-viral recognition•Antibody somatic hypermutation is a major determinant for HIV-1/HCV cross-reactivity Pilewski et al. uses LIBRA-seq to identify multiple, functional HIV-1/HCV cross-reactive antibodies with diverse epitope specificities. This study identifies a glycan-reactive, broadly anti-viral antibody recognizing the NTD of SARS-CoV-2, in addition to antigens from multiple other viruses. The authors further show that somatic hypermutation both establishes and enhances HIV-1/HCV cross-reactivity.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor

United States: Elsevier Inc

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