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Fecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing Enterobacteriaceae in Egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study

Abdallah, H M ; Alnaiemi, N ; Reuland, E A ; Wintermans, B B ; Koek, A ; Abdelwahab, A M ; Samy, A ; Abdelsalam, K W ; Vandenbroucke-Grauls, C M J E

Antimicrobial resistance & infection control, 2017-06, Vol.6 (1), p.62-62, Article 62 [Periódico revisado por pares]

England: BioMed Central Ltd

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  • Título:
    Fecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing Enterobacteriaceae in Egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study
  • Autor: Abdallah, H M ; Alnaiemi, N ; Reuland, E A ; Wintermans, B B ; Koek, A ; Abdelwahab, A M ; Samy, A ; Abdelsalam, K W ; Vandenbroucke-Grauls, C M J E
  • Assuntos: Antibiotics ; Antimicrobial agents ; Automation ; Bacteria ; Beta lactamases ; Carbapenemase-ESBL-Egypt-resistance-Enterobacteriaceae ; Carbapenems ; Cross-sectional studies ; Deoxyribonucleic acid ; Disease control ; DNA ; Drug resistance ; Drug resistance in microorganisms ; E coli ; Enterobacter ; Enterobacteriaceae ; Enzymes ; Feces ; Genetic aspects ; Health aspects ; Infections ; Internet ; Studies
  • É parte de: Antimicrobial resistance & infection control, 2017-06, Vol.6 (1), p.62-62, Article 62
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints. The fecal samples were plated directly on selective EbSA-ESBL Screening Agar and on MacConkey agar. Isolate identification was performed with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Screening for ESBLs and carbapenemases production was done by both the automated VITEK®2 system with AST N198 and by disk diffusion method. Real-time PCR and sequencing were used to characterize the resistance genes. Phylogroups of the isolates were determined by a triplex PCR-based method. Of 100 patients screened for fecal colonization with extended-spectrum β-lactamase -producing Enterobacteriaceae (ESBL-E) and carbapenemase- producing Enterobacteriaceae (CPE), 68 were colonized with ESBL-E whereas five patients were positive for CPE. One hundred and thirteen Enterobacterceae isolates were recovered from 100 fecal samples, they belonged to (  = 72), (  = 23), (  = 3), spp. (  = 1) and other Enterobacterceae isolates (  = 14). The gene was detected in 89.04% (65/73) of the ESBL-producing Enterobacteriaceae, whereas and were detected in 30.14% (22/73) and 19.18% (14/73) respectively. Three out of 5 carbapenem-resistant isolates harbored New Delhi metallo-beta-lactamase (NDM) and 2 produced Verona integron-encoded metallo- beta -lactamase (VIM). Twenty-two (47.83%) of the ESBL positive isolates were multidrug resistant (MDR). Phylogenetic analysis showed that, of the 51 ESBL-EC isolates, 17 belonged to group B2, 13 to group D, 11 to group A and 10 to group B1. Nearly two-thirds of the Enterobacteriaceae isolates recovered from feces of ambulatory patients with community-onset gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt were ESBL producers and one in every 20 patients included in our study was colonized by carbapenemase-producing Enterobacteriaceae. These high colonization rates are worrying, therefore prudent antimicrobial use should be adopted in Egyptian community settings.
  • Editor: England: BioMed Central Ltd
  • Idioma: Inglês
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