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Effects of freezing/thawing on the mechanical properties of decellularized lungs

Nonaka, Paula N. ; Campillo, Noelia ; Uriarte, Juan J. ; Garreta, Elena ; Melo, Esther ; de Oliveira, Luis V. F. ; Navajas, Daniel ; Farré, Ramon

Journal of biomedical materials research. Part A, 2014-02, Vol.102 (2), p.413-419 [Periódico revisado por pares]

Hoboken, NJ: Blackwell Publishing Ltd

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  • Título:
    Effects of freezing/thawing on the mechanical properties of decellularized lungs
  • Autor: Nonaka, Paula N. ; Campillo, Noelia ; Uriarte, Juan J. ; Garreta, Elena ; Melo, Esther ; de Oliveira, Luis V. F. ; Navajas, Daniel ; Farré, Ramon
  • Assuntos: Animals ; Biological and medical sciences ; elastance ; Female ; Freezing ; freezing/thawing ; Lung - chemistry ; lung bioengineering ; lung decellularization ; mechanical ventilation ; Medical sciences ; Mice ; organ scaffold ; Sodium Dodecyl Sulfate - chemistry ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Tissue Scaffolds - chemistry
  • É parte de: Journal of biomedical materials research. Part A, 2014-02, Vol.102 (2), p.413-419
  • Notas: Spanish Ministry of Economy and Competitiveness - No. SAF2011-22576, FIS-PI11/00089
    istex:0E52765E0483FE45F6EAFCA911DFE1759B480306
    ark:/67375/WNG-75HL5H29-Z
    Fundação de Amparo à Pesquisa do Estado de São Paulo - No. 2012/04052-2 (Fellowship)
    ArticleID:JBMA34708
    The Conselho Nacional de Desenvolvimento Científico e Tecnológico - No. Research Productivity modality 307618/2010-2
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Lung bioengineering based on decellularized organ scaffolds is a potential alternative for transplantation. Freezing/thawing, a usual procedure in organ decellularization and storage could modify the mechanical properties of the lung scaffold and reduce the performance of the bioengineered lung when subjected to the physiological inflation‐deflation breathing cycles. The aim of this study was to determine the effects of repeated freezing/thawing on the mechanical properties of decellularized lungs in the physiological pressure–volume regime associated with normal ventilation. Fifteen mice lungs (C57BL/6) were decellularized using a conventional protocol not involving organ freezing and based on sodium dodecyl sulfate detergent. Subsequently, the mechanical properties of the acellular lungs were measured before and after subjecting them to three consecutive cycles of freezing/thawing. The resistance (RL) and elastance (EL) of the decellularized lungs were computed by linear regression fitting of the recorded signals (tracheal pressure, flow, and volume) during mechanical ventilation. RL was not significantly modified by freezing‐thawing: from 0.88 ± 0.37 to 0.90 ± 0.38 cmH2O·s·mL−1 (mean ± SE). EL slightly increased from 64.4 ± 11.1 to 73.0 ± 16.3 cmH2O·mL−1 after the three freeze‐thaw cycles (p = 0.0013). In conclusion, the freezing/thawing process that is commonly used for both organ decellularization and storage induces only minor changes in the ventilation mechanical properties of the organ scaffold. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 413–419, 2014.
  • Editor: Hoboken, NJ: Blackwell Publishing Ltd
  • Idioma: Inglês
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