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A Secreted Factor Coordinates Environmental Quality with Bacillus Development

Ababneh, Qutaiba O ; Tindall, Amanda J ; Herman, Jennifer K Driks, Adam

PloS one, 2015-12, Vol.10 (12), p.e0144168-e0144168 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A Secreted Factor Coordinates Environmental Quality with Bacillus Development
Autor: Ababneh, Qutaiba O ; Tindall, Amanda J ; Herman, Jennifer K
Driks, Adam
Assuntos: Alleles ; Bacillus ; Bacillus (Bacteria) ; Bacillus - drug effects ; Bacillus - growth & development ; Bacillus - metabolism ; Bacillus subtilis ; Bacteria ; Bacterial Proteins - metabolism ; Bacterial spores ; Biochemistry ; Biophysics ; Cell division ; Culture Media, Conditioned - pharmacology ; Deoxyribonucleic acid ; Dialysis ; DNA ; Endopeptidase K ; Endopeptidase K - metabolism ; Environment ; Environmental aspects ; Environmental quality ; Enzymes ; Gene expression ; Genetic aspects ; Kinases ; Membrane Transport Proteins - metabolism ; Membranes, Artificial ; Molecular Weight ; Peptides ; Peptides - metabolism ; Phosphatase ; Phosphorylation ; Physiological aspects ; Proteinase ; Signal transduction ; Spores (Bacteria) ; Spores, Bacterial - drug effects ; Spores, Bacterial - metabolism ; Sporulation ; Stationary phase ; Transporter
É parte de: PloS one, 2015-12, Vol.10 (12), p.e0144168-e0144168
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: QOA AJT JKH. Performed the experiments: QOA AJT JKH. Analyzed the data: QOA AJT JKH. Contributed reagents/materials/analysis tools: QOA AJT JKH. Wrote the paper: QOA AJT JKH.
Descrição: Entry into sporulation is governed by the master regulator Spo0A. Spo0A accumulates in its active form, Spo0A-P, as cells enter stationary phase. Prior reports have shown that the acute induction of constitutively active Spo0A during exponential growth does not result in sporulation. However, a subsequent study also found that a gradual increase in Spo0A-P, mediated through artificial expression of the kinase, KinA, during exponential growth, is sufficient to trigger sporulation. We report here that sporulation via KinA induction depends on the presence of an extracellular factor or factors (FacX) that only accumulates to active levels during post-exponential growth. FacX is retained by dialysis with a cutoff smaller than 500 Dalton, can be concentrated, and is susceptible to proteinase K digestion, similar to described quorum-sensing peptides shown to be involved in promoting sporulation. However, unlike previously characterized peptides, FacX activity does not require the Opp or App oligopeptide transporter systems. In addition, FacX activity does not depend on SigH, Spo0A, or ComX. Importantly, we find that in the presence of FacX, B. subtilis can be induced to sporulate following the artificial induction of constitutively active Spo0A. These results indicate that there is no formal requirement for gradual Spo0A-P accumulation and instead support the idea that sporulation requires both sufficient levels of active Spo0A and at least one other signal or condition.
Editor: United States: Public Library of Science
Idioma: Inglês

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A Secreted Factor Coordinates Environmental Quality with Bacillus Development

Ababneh, Qutaiba O ; Tindall, Amanda J ; Herman, Jennifer K Driks, Adam

United States: Public Library of Science

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