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Hamster DAX1: Molecular insights, specific expression, and its role in the Harderian gland

Ramos, L. ; Mares, L.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 2022-01, Vol.263, p.111096-111096, Article 111096 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Hamster DAX1: Molecular insights, specific expression, and its role in the Harderian gland
  • Autor: Ramos, L. ; Mares, L.
  • Assuntos: Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; DAX-1 Orphan Nuclear Receptor - chemistry ; DAX-1 Orphan Nuclear Receptor - genetics ; DAX-1 Orphan Nuclear Receptor - metabolism ; DAX1 ; Female ; Harderian gland ; Harderian Gland - metabolism ; Male ; Mesocricetus - genetics ; Mesocricetus - metabolism ; Models, Molecular ; Phylogeny ; Sequence Homology, Amino Acid ; Sex development ; Sexual dimorphism ; Transcriptome
  • É parte de: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 2022-01, Vol.263, p.111096-111096, Article 111096
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: DAX1 plays an essential role in the differentiation and physiology of the Hypothalamic-Pituitary-Adrenal-Gonadal (HPAG) axis during embryogenesis. However, in adult tissues, in addition to the HPAG axis, evidence has not been found for its differential expression and function. We isolated the DAX1 cDNA to analyze its tissue localization and gene expression profiles in male and female hamsters' Harderian glands (HGs), Mesocricetus auratus. The isolated cDNA clone contains 1848 base pairs (bp), and a 1428-bp open reading frame (ORF) encodes a 476 amino acid protein. Sequence alignments and the phylogenetic tree display a relevant percentage of similarity with human (66%), rat (81%), and mouse (84%) sequences. In adult tissues, the mRNA distribution demonstrated that DAX1 is present in testis, ovaries, and male and female HGs. The highest expression profiles were identified in the adrenal glands, where females exhibit higher mRNA levels than males. The sexually dimorphic expression of DAX1 in adrenals suggests that its presence could be associated with regulating, functioning, and maintaining this endocrine tissue. These findings indicate that the DAX1 gene is limitedly expressed in adult tissues. In the HGs, we demonstrate the absence of sexually dimorphic gene expression. Our results suggest that DAX1 might have an additional physiological function outside of the HPAG axis, specifically in the HG, which may be required for the regulation of intracrine steroidogenesis, secretion, and maintenance of exocrine tissue. [Display omitted] •Full-length nucleotide sequence of hamster DAX1 was isolated and cloned•Hamster DAX1 displayed conserved domains•DAX1 lacked sexually dimorphic expression in hamster HG
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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