skip to main content
Idioma:

Evaluation of cell damage and modulation of cytokines TNF-α, IL-6 and IL-10 in macrophages exposed to PpIX-mediated photodynamic therapy

R. D. R. Tiveron ; D. A. Costa ; M. D. I. Leite ; C. B. S. Vaz ; M. Sousa ; S. M. C. F. Carlos ; C. J. F. Oliveira ; R. R. Machado ; T. P. Paulino

SciELO journals 2019

Texto completo disponível

Citações Citado por
  • Título:
    Evaluation of cell damage and modulation of cytokines TNF-α, IL-6 and IL-10 in macrophages exposed to PpIX-mediated photodynamic therapy
  • Autor: R. D. R. Tiveron ; D. A. Costa ; M. D. I. Leite ; C. B. S. Vaz ; M. Sousa ; S. M. C. F. Carlos ; C. J. F. Oliveira ; R. R. Machado ; T. P. Paulino
  • Assuntos: FOS: Biological sciences ; Microbiology not elsewhere classified
  • Notas: RelationTypeNote: IsSupplementTo -- 10.1590/1519-6984.193748
    10.1590/1519-6984.193748
  • Descrição: Abstract Little is known regarding whether photodynamic therapy (PDT)-induced cell death can substantially compromise macrophages (MΦ), which are important cells in PDT-induced immune responses. Here, parameters of PDT-mediated MΦ cytotoxicity and cytokine production in response to protoporphyrin IX (PpIX) were evaluated. Peritoneal MΦ from BALB/c mice were stimulated in vitro with PDT, light, PpIX, or lipopolysaccharide (LPS). After that, cell viability, lipid peroxidation, Nitric Oxide (NO), DNA damage, TNF-α, IL-6 and IL-10 were evaluated. Short PDT exposure reduced cell viability by 10–30%. There was a two-fold increase in NO and DNA degradation, despite the non-increase in lipoperoxidation. PDT increased TNF-α and IL-10, particularly in the presence of LPS, and decreased the production of IL-6 to 10-fold. PDT causes cellular stress, induces NO radicals and leads to DNA degradation, generating a cytotoxic microenvironment. Furthermore, PDT modulates pro- and anti-inflammatory cytokines in MΦ.
  • Editor: SciELO journals
  • Data de criação/publicação: 2019
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  3 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.