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A novel cationic liposome reagent for efficient transfection of mammalian cells

Gao, Xiang ; Huang, Leaf

Biochemical and biophysical research communications, 1991-08, Vol.179 (1), p.280-285 [Periódico revisado por pares]

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Título:
A novel cationic liposome reagent for efficient transfection of mammalian cells
Autor: Gao, Xiang ; Huang, Leaf
Assuntos: Animals ; Biological and medical sciences ; Cell Line ; Cell Survival - drug effects ; Chloramphenicol O-Acetyltransferase - genetics ; Chloramphenicol O-Acetyltransferase - metabolism ; Cholesterol - analogs & derivatives ; Cholesterol - chemical synthesis ; Diverse techniques ; DNA - analysis ; Fundamental and applied biological sciences. Psychology ; Humans ; Indicators and Reagents ; Liposomes ; Mammals ; Molecular and cellular biology ; Phosphatidylethanolamines - pharmacology ; Transfection
É parte de: Biochemical and biophysical research communications, 1991-08, Vol.179 (1), p.280-285
Descrição: A novel cationic derivative of cholesterol, 3β[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine. This novel cationic liposome reagent facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells, A549 human lung carcinoma cells, L929 mouse fibroblast cells, and YPT minipig primary endothelial cells. The activity was greater than that of a commercial reagent, Lipofectin, and was approximately 4-fold less toxic than Lipofectin when assayed with A431 cells. The reagent is easy to synthesize and stable for at least 6 weeks.
Editor: San Diego, CA: Elsevier Inc
Idioma: Inglês

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