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Global response of Saccharomyces cerevisiae to an alkylating agent

Jelinsky, S.A. (Harvard School of Public Health, Boston, MA.) ; Samson, L.D

Proceedings of the National Academy of Sciences - PNAS, 1999-02, Vol.96 (4), p.1486-1491 [Periódico revisado por pares]

United States: National Academy of Sciences of the United States of America

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Título:
Global response of Saccharomyces cerevisiae to an alkylating agent
Autor: Jelinsky, S.A. (Harvard School of Public Health, Boston, MA.) ; Samson, L.D
Assuntos: ADN ; Alkylating Agents - pharmacology ; ANALYTICAL METHODS ; ARN MENSAJERO ; ARN MESSAGER ; BIOCHEMISTRY ; BIOCHIMIE ; Biological Sciences ; BIOQUIMICA ; COMPLEMENTARY RNA HYBRIDIZATION ; Dehydrogenases ; DNA ; DNA CHIP ANALYSIS ; DNA Damage ; DNA MODIFICATION ; DNA repair ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fungal Proteins - genetics ; GENE ; GENE EXPRESSION ; GENECHIP HYBRIDIZATION ; GENES ; Genes, Fungal ; GENETIC ANALYSIS ; GENETICA ; GENETICS ; GENETIQUE ; GENOMAS ; GENOME ; Genome, Fungal ; GENOMES ; MESSENGER RNA ; METHODE ; METHODS ; METHYL METHANESULFONATE ; Methyl Methanesulfonate - pharmacology ; METODOS ; MMS ; NORTHERN BLOTTING ; NUCLEOTIDE SEQUENCE ; OPEN READING FRAMES ; Proteins ; Ribosomal proteins ; RNA ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; Transcription, Genetic - drug effects
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1999-02, Vol.96 (4), p.1486-1491
Notas: 1999004549
F30
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
To whom reprint requests should be addressed. e-mail: lsamson@sph.harvard.edu.
Edited by David Botstein, Stanford University School of Medicine, Stanford, CA, and approved December 7, 1998
Descrição: DNA chip technology enables simultaneous examination of how approximately 6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change. By using chips bearing oligonucleotide arrays, we show that, after exposure to the alkylating agent methyl methanesulfonate, approximately 325 gene transcript levels are increased and approximately 76 are decreased. Of the 21 genes that already were known to be induced by a DNA-damaging agent, 18 can be scored as inducible in this data set, and surprisingly, most of the newly identified inducible genes are induced even more strongly than these 18. We examined 42 responsive and 8 nonresponsive ORFs by conventional Northern blotting, and 48 of these 50 ORFs responded as they did by DNA chip analysis, with magnitudes displaying a correlation coefficient of 0.79. Responsive genes fall into several expected and many unexpected categories. Evidence for the induction of a program to eliminate and replace alkylated proteins is presented
Editor: United States: National Academy of Sciences of the United States of America
Idioma: Inglês

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Global response of Saccharomyces cerevisiae to an alkylating agent

Jelinsky, S.A. (Harvard School of Public Health, Boston, MA.) ; Samson, L.D

United States: National Academy of Sciences of the United States of America

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