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Microscopic and molecular characterization of ovarian follicle atresia in Rhodnius prolixus Stahl under immune challenge

Medeiros, Marcelo N ; Ramos, Isabela B ; Oliveira, Danielle M.P ; Silva, Rodrigo C.B. da ; Gomes, Fabio M ; Medeiros, Luciano N ; Kurtenbach, Eleonora ; Chiarini, Luciana B ; Masuda, Hatisaburo ; Souza, Wanderley de ; Machado, Ednildo A

Journal of insect physiology, 2011-07, Vol.57 (7), p.945-953 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Microscopic and molecular characterization of ovarian follicle atresia in Rhodnius prolixus Stahl under immune challenge
Autor: Medeiros, Marcelo N ; Ramos, Isabela B ; Oliveira, Danielle M.P ; Silva, Rodrigo C.B. da ; Gomes, Fabio M ; Medeiros, Luciano N ; Kurtenbach, Eleonora ; Chiarini, Luciana B ; Masuda, Hatisaburo ; Souza, Wanderley de ; Machado, Ednildo A
Assuntos: abnormal development ; acidification ; adults ; Animals ; Apoptosis ; Aspartic Acid Proteases - metabolism ; Aspergillus niger ; Aspergillus niger - physiology ; Cathepsins ; chromatin ; conidia ; Cysteine Proteases - metabolism ; egg yolk ; eggs ; epithelium ; Female ; females ; Fluorescent Dyes ; Follicle atresia ; Follicular Atresia ; fungi ; hemocoel ; In Situ Nick-End Labeling ; Indoles - chemistry ; insects ; light microscopy ; longevity ; Microscopy, Electron, Transmission ; mitochondria ; ovarian follicles ; Programmed cell death ; protein content ; proteinases ; resorption ; Rhodnius - immunology ; Rhodnius - microbiology ; Rhodnius - physiology ; Rhodnius prolixus ; shrinkage ; transmission electron microscopy ; vacuoles ; Vitellogenesis
É parte de: Journal of insect physiology, 2011-07, Vol.57 (7), p.945-953
Notas: http://dx.doi.org/10.1016/j.jinsphys.2011.04.010
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Descrição: In this work we characterized the degenerative process of ovarian follicles of the bug Rhodnius prolixus challenged with the non-entomopathogenic fungus Aspergillus niger. An injection of A. niger conidia directly into the hemocoel of adult R. prolixus females at the onset of vitellogenesis caused no effect on host lifespan but elicited a net reduction in egg batch size. Direct inspection of ovaries from the mycosed insects revealed that fungal challenge led to atresia of the vitellogenic follicles. Light microscopy and DAPI staining showed follicle shrinkage, ooplasm alteration and disorganization of the monolayer of follicle cells in the atretic follicles. Transmission electron microscopy of thin sections of follicle epithelium also showed nuclei with condensed chromatin, electron dense mitochondria and large autophagic vacuoles. Occurrence of apoptosis of follicle cells in these follicles was visualized by TUNEL labeling. Resorption of the yolk involved an increase in protease activities (aspartyl and cysteinyl proteases) which were associated with precocious acidification of yolk granules and degradation of yolk protein content. The role of follicle atresia in nonspecific host–pathogen associations and the origin of protease activity that led to yolk resorption are discussed.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Microscopic and molecular characterization of ovarian follicle atresia in Rhodnius prolixus Stahl under immune challenge

Medeiros, Marcelo N ; Ramos, Isabela B ; Oliveira, Danielle M.P ; Silva, Rodrigo C.B. da ; Gomes, Fabio M ; Medeiros, Luciano N ; Kurtenbach, Eleonora ; Chiarini, Luciana B ; Masuda, Hatisaburo ; Souza, Wanderley de ; Machado, Ednildo A

England: Elsevier Ltd

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