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Expression of genes related to tropism in regenerating slow-twitch muscles of rats treated with cyclosporin A

Elen Haruka Miyabara M. S Aoki; Anselmo Sigari Moriscot; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (19. 2004 Águas de Lindóia, SP)

Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2004

Águas de Lindóia 2004

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  • Título:
    Expression of genes related to tropism in regenerating slow-twitch muscles of rats treated with cyclosporin A
  • Autor: Elen Haruka Miyabara
  • M. S Aoki; Anselmo Sigari Moriscot; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (19. 2004 Águas de Lindóia, SP)
  • Assuntos: HISTOLOGIA
  • É parte de: Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2004
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Objetivo: Skeletal muscle regeneration is a complex and finely orchestrated set of cellular and molecular responses. Recently, it has been described that calcineurin, a Ca++/calmodulin dependent phosphatase, is involved in skeletal muscle regeneration (Acta Neuropathol 105:271, 2003). This work was undertaken to study the expression of genes related to tropism (myostatin, calpain-3 and atrogin-1) in regenerating slow-twitch muscles of rats treated with cyclosporin-A (CsA), a calcineurin inhibitor. Métodos e Resultados: Rats (n=36) weighing 230-300 g were treated with CsA for 5 days. On the 6th day, animals were submitted to cryolesion of the left soleus muscles and were sacrificed 1, 10 and 21 days after cryolesion. Soleus muscles were removed, frozen and stored in liquid nitrogen. Body and muscle weights and histological sections stained with toluidine blue were obtained to confirm that cryolesion and CsA treatment were effective. Gene expression was determined by quantitative real time PCR and results demonstrate that atrogin-1 gene expression is decreased at 1 day after cryolesion and cryolesion + CsA treatment (0.5±0.15 and 0.7±0.14 vs control (C): 1±0.2 fold, respectively). Calpain-3 gene expression at 1 day of regeneration is repressed, in both CsA treated and non-treated groups (0.12±0.1 and 0.35±0.04 vs C: 1±0.1 fold, respectively). Calpain-3 gene expression is strongly upregulated (5.2±0.1 vs C: 1,1±0.3 fold) at 10 days after cryolesion.
    Interestingly, CsA blocks calpain-3 gene expression rise induced by cryolesion in this same period. Myostatin gene expression is not altered among all groups. Conclusões: The results show that atrogin-1 might be involved in the early stages of regeneration independently of calcineurin and calpain-3 might have a differential role in the early and late stages of regeneration in a calcineurin-dependent manner
  • Editor: Águas de Lindóia
  • Data de criação/publicação: 2004
  • Formato: res. 10.039.
  • Idioma: Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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