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Brief Report: The lincRNA Hotair Is Required for Epithelial‐to‐Mesenchymal Transition and Stemness Maintenance of Cancer Cell Lines

Pádua Alves, Cleidson ; Fonseca, Aline Simoneti ; Muys, Bruna Rodrigues ; Barros e Lima Bueno, Rafaela ; Bürger, Matheus Carvalho ; Souza, Jorge E. S. ; Valente, Valeria ; Zago, Marco Antonio ; Silva, Wilson Araújo

Stem cells (Dayton, Ohio), 2013-12, Vol.31 (12), p.2827-2832 [Periódico revisado por pares]

United States: Oxford University Press

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  • Título:
    Brief Report: The lincRNA Hotair Is Required for Epithelial‐to‐Mesenchymal Transition and Stemness Maintenance of Cancer Cell Lines
  • Autor: Pádua Alves, Cleidson ; Fonseca, Aline Simoneti ; Muys, Bruna Rodrigues ; Barros e Lima Bueno, Rafaela ; Bürger, Matheus Carvalho ; Souza, Jorge E. S. ; Valente, Valeria ; Zago, Marco Antonio ; Silva, Wilson Araújo
  • Assuntos: Breast cancer ; Cancer stem cell ; Cell Line, Tumor ; Epithelial-Mesenchymal Transition - genetics ; Epithelial‐to‐mesenchymal transition ; Hotair ; Humans ; LincRNA ; Neoplastic Stem Cells - pathology ; Neoplastic Stem Cells - physiology ; RNA, Long Noncoding - genetics ; Stem cells ; Transfection
  • É parte de: Stem cells (Dayton, Ohio), 2013-12, Vol.31 (12), p.2827-2832
  • Notas: Author contributions: C.A.: conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing; A.F., B.M., and R.B.: collection and/or assembly of data; M.C.B. and J.E.S.d.S.: data analysis and interpretation; V.V.: conception and design, data analysis and interpretation, and manuscript writing; M.Z.: financial support and manuscript writing; W.A.S.: conception and design, financial support, data analysis and interpretation, manuscript writing, and final approval of manuscript.
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  • Descrição: Hotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin‐modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial‐to‐mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF‐β1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF‐β1, and also the colony‐forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD133+/CD44+) presents much higher levels of Hotair when compared with the non‐stem cell subpopulation. These results indicate that Hotair acts as a key regulator that controls the multiple signaling mechanisms involved in EMT. Altogether, our data suggest that the role of Hotair in tumorigenesis occurs through EMT triggering and stemness acquisition. Stem Cells 2013;31:2827–2832
  • Editor: United States: Oxford University Press
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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