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Dynamics and conformational studies of TOAC spin labeled analogues of Ctx(Ile(21))-Ha peptide from Hypsiboas albopunctatus

Vicente, Eduardo F ; Basso, Luis Guilherme M ; Cespedes, Graziely F ; Lorenzón, Esteban N ; Castro, Mariana S ; Mendes-Giannini, Maria José S ; Costa-Filho, Antonio José ; Cilli, Eduardo M van Raaij, Mark J.

PloS one, 2013-04, Vol.8 (4), p.e60818-e60818 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Dynamics and conformational studies of TOAC spin labeled analogues of Ctx(Ile(21))-Ha peptide from Hypsiboas albopunctatus
Autor: Vicente, Eduardo F ; Basso, Luis Guilherme M ; Cespedes, Graziely F ; Lorenzón, Esteban N ; Castro, Mariana S ; Mendes-Giannini, Maria José S ; Costa-Filho, Antonio José ; Cilli, Eduardo M
van Raaij, Mark J.
Assuntos: Amino Acid Sequence ; Amino acids ; Amphibian Proteins - analysis ; Amphibian Proteins - chemistry ; Animals ; Antibiotics ; Antimicrobial agents ; Antimicrobial Cationic Peptides - analysis ; Antimicrobial Cationic Peptides - chemistry ; Antimicrobial peptides ; Anura - metabolism ; Biochemistry ; Biological activity ; Biology ; Biopolymers ; Camps ; Carboxylic acids ; Cell Membrane - chemistry ; Cell membranes ; Chemistry ; Circular Dichroism ; Conformation ; Cyclic N-Oxides ; Dichroism ; Electron paramagnetic resonance ; Electron spin ; Electron spin resonance ; Electron Spin Resonance Spectroscopy ; Fluorescence ; Hydrophobic and Hydrophilic Interactions ; Hydrophobicity ; Immobilization ; Lysophosphatidylcholines ; Membranes ; Membranes, Artificial ; Micelles ; Molecular Dynamics Simulation ; Molecular Sequence Data ; Peptides ; Phospholipids ; Polar regions ; Protein structure ; Protein Structure, Secondary ; Secondary structure ; Spectrum analysis ; Spin dynamics ; Spin Labels ; Spin resonance ; Studies ; Topology ; Trifluoroethanol
É parte de: PloS one, 2013-04, Vol.8 (4), p.e60818-e60818
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: EMC AJCF EFV LGMB. Performed the experiments: EFV ENL LGMB EFV GFC MJSMG MSC. Analyzed the data: EMC AJCF EFV LGMB MSC. Contributed reagents/materials/analysis tools: EMC AJCF EFV LGMB MJSMG. Wrote the paper: EMC AJCF EFV LGMB.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Antimicrobial peptides (AMPs) isolated from several organisms have been receiving much attention due to some specific features that allow them to interact with, bind to, and disrupt cell membranes. The aim of this paper was to study the interactions between a membrane mimetic and the cationic AMP Ctx(Ile(21))-Ha as well as analogues containing the paramagnetic amino acid 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) incorporated at residue positions n = 0, 2, and 13. Circular dichroism studies showed that the peptides, except for [TOAC(13)]Ctx(Ile(21))-Ha, are unstructured in aqueous solution but acquire different amounts of α-helical secondary structure in the presence of trifluorethanol and lysophosphocholine micelles. Fluorescence experiments indicated that all peptides were able to interact with LPC micelles. In addition, Ctx(Ile(21))-Ha and [TOAC(13)]Ctx(Ile(21))-Ha peptides presented similar water accessibility for the Trp residue located near the N-terminal sequence. Electron spin resonance experiments showed two spectral components for [TOAC(0)]Ctx(Ile(21))-Ha, which are most likely due to two membrane-bound peptide conformations. In contrast, TOAC(2) and TOAC(13) derivatives presented a single spectral component corresponding to a strong immobilization of the probe. Thus, our findings allowed the description of the peptide topology in the membrane mimetic, where the N-terminal region is in dynamic equilibrium between an ordered, membrane-bound conformation and a disordered, mobile conformation; position 2 is most likely situated in the lipid polar head group region, and residue 13 is fully inserted into the hydrophobic core of the membrane.
Editor: United States: Public Library of Science
Idioma: Inglês

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Dynamics and conformational studies of TOAC spin labeled analogues of Ctx(Ile(21))-Ha peptide from Hypsiboas albopunctatus

Vicente, Eduardo F ; Basso, Luis Guilherme M ; Cespedes, Graziely F ; Lorenzón, Esteban N ; Castro, Mariana S ; Mendes-Giannini, Maria José S ; Costa-Filho, Antonio José ; Cilli, Eduardo M van Raaij, Mark J.

United States: Public Library of Science

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