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Spheroplast and protoplast formation from cysts of Phytophthora sojae treated with digestive enzymes

Wagner, R.E. (University of Illinois, Urbana, IL) ; Wilkinson, H.T

Mycologia, 1993-01, Vol.85 (1), p.131-136 [Periódico revisado por pares]

Lawrence, KS: Taylor & Francis

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Título:
Spheroplast and protoplast formation from cysts of Phytophthora sojae treated with digestive enzymes
Autor: Wagner, R.E. (University of Illinois, Urbana, IL) ; Wilkinson, H.T
Assuntos: AGENT PATHOGENE ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Brief Article ; CHAMPIGNON ; CHEMICAL DEGRADATION ; Cysts ; DEGRADACION ; DEGRADATION ; Digestive enzymes ; ENZIMAS ; ENZYME ; ENZYMES ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; FUNGI ; Glycine max ; HONGOS ; ISOLATION TECHNIQUES ; Magnesium ; Microbial genetics ; Mycelium ; ORGANISMOS PATOGENOS ; OSMOTIC PRESSURE ; P. megasperma ; PATHOGENS ; Phytopathology ; Phytopathology. Animal pests. Plant and forest protection ; PHYTOPHTHORA ; Phytophthora sojae ; PLANT PATHOGENIC FUNGI ; PRESION OSMOTICA ; PRESSION OSMOTIQUE ; PROTOPLASTE ; PROTOPLASTOS ; PROTOPLASTS ; Spheroplasts ; TECHNIQUE DE L'ISOLEMENT ; TECNICAS DE AISLAMIENTO ; Variation, races, biotypes, parasitic specialization, genetics ; zoospores
É parte de: Mycologia, 1993-01, Vol.85 (1), p.131-136
Notas: 9401157
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ObjectType-Article-2
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Descrição: Protoplasts and spheroplasts are useful tools for studying the genetics of fungi, especially Phytophthora sojae. The purpose of this investigation was to produce spheroplasts and protoplasts from cysts of P. sojae with and without magnesium as the osmotic stabilizer. Factors evaluated for spheroplast and protoplast formation from cysts include: type and concentration of digestive enzyme; type and concentration of osmotic stabilizer; preenzyme incubation in detergent or thiol compounds; and reduction of the osmotic potential following incubation in digestive enzymes.
Editor: Lawrence, KS: Taylor & Francis
Idioma: Inglês

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Spheroplast and protoplast formation from cysts of Phytophthora sojae treated with digestive enzymes

Wagner, R.E. (University of Illinois, Urbana, IL) ; Wilkinson, H.T

Lawrence, KS: Taylor & Francis

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