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Dental cementum examination with SEM/EDX for unraveling details along the lifeline to assist identification of a female individual

Ristova, Mimoza ; Josheva, Dijana ; Brozek-Mucha, Zuzana

Forensic science international, 2022-01, Vol.330, p.111103-111103, Article 111103 [Periódico revisado por pares]

Ireland: Elsevier B.V

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  • Título:
    Dental cementum examination with SEM/EDX for unraveling details along the lifeline to assist identification of a female individual
  • Autor: Ristova, Mimoza ; Josheva, Dijana ; Brozek-Mucha, Zuzana
  • Assuntos: Age ; age scale ; Archaeology ; Breast feeding ; Ca and P contents ; Calcium ; Cementum ; Childbirth & labor ; childbirths ; Dental cement ; Dental Cementum ; Emission analysis ; Female ; Females ; Forensic science ; Forensic sciences ; Human remains ; Humans ; incremental lines ; Metabolism ; Microscopy, Electron, Scanning ; Nutrition ; Odontology ; Phosphorus ; Pregnancy ; Qualitative analysis ; Scanning electron microscopy ; SEM-EDX ; Teeth
  • É parte de: Forensic science international, 2022-01, Vol.330, p.111103-111103, Article 111103
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
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  • Descrição: In this work, we present a novel approach of forensic examination of the dental cementum by Scanning Electron Microscopy (SEM) as an imaging tool, complemented by semiquantitative elemental analysis of the incremental lines with an Energy Dispersion X-ray analyzer. Tooth samples were collected from female donors who are known to have given single or multiple childbirths. This research is aimed to prove the following hypothesis - if pregnancy (possibly followed by breastfeeding) affects the calcium and phosphorus levels in female metabolism, then increased levels of Ca and P should be detected in the dental cementum region corresponding to the age of the pregnancy. For this purpose, EDX spectra were taken from sampling points, evenly aligned along the direction of the cementum growth. The most intensive X-ray peaks were analyzed employing qualitative and semiquantitative elemental analysis. Results showed that the changes in the peak values of Ca and P levels in the cementum along the lifeline could be related to the biological age of pregnancy, possibly followed by breastfeeding. The observed variations in other elements could also suggest some other physiological conditions or nutrition patterns. These preliminary results could trigger more profound and extensive research involving more sophisticated techniques for elemental analysis, such as PIXE (Proton Induced X-ray Emission). Correlation of the element levels to certain conditions may bring a new paradigm of the unique forensic value of the dental cementum, on which the life story of a person is literally "written in a stone". This method could serve pure forensic purposes and archeological examinations of human findings in support of historical facts. Using SEM/EDX on the dental cementum to detect childbirth of a female individual. [Display omitted] •Childbirth could be detected through increased Ca and P levels in the dental cementum.•Age scale is created in the SEM images in the growth direction of the dental cementum.•EDX spectra are used for elemental level variations in the dental cementum•Elements' variations in dental cementum could indicate some physiological conditions.•This method could be applied for forensic/archeological examinations of human findings.
  • Editor: Ireland: Elsevier B.V
  • Idioma: Inglês

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