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Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity

Kuribayashi, Megumi ; Kitasako, Yuichi ; Matin, Khairul ; Sadr, Alireza ; Shida, Kanako ; Tagami, Junji

Journal of dentistry, 2012-03, Vol.40 (3), p.222-228 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity
Autor: Kuribayashi, Megumi ; Kitasako, Yuichi ; Matin, Khairul ; Sadr, Alireza ; Shida, Kanako ; Tagami, Junji
Assuntos: Acidity ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bacteria ; Bacterial Load ; Caries ; Cariogenic bacteria ; Dental Caries - classification ; Dental Caries - microbiology ; Dental Caries - physiopathology ; Dental Caries Activity Tests - instrumentation ; Dental Caries Activity Tests - methods ; Dentin - microbiology ; Dentin - physiopathology ; Dentistry ; DMF Index ; DNA, Bacterial - analysis ; Electrodes ; Genome, Bacterial - genetics ; Humans ; Hydrogen-Ion Concentration ; Hypotheses ; Lactobacillus ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Methods ; Micro-pH sensor ; Middle Aged ; Mouth - physiology ; Patient education ; qPCR ; Real-Time Polymerase Chain Reaction ; Risk assessment ; Semiconductors ; Streptococcus mutans - genetics ; Streptococcus mutans - isolation & purification ; Streptococcus sobrinus - genetics ; Streptococcus sobrinus - isolation & purification ; Studies ; Tooth Remineralization ; Young Adult
É parte de: Journal of dentistry, 2012-03, Vol.40 (3), p.222-228
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Descrição: Abstract Objectives A low pH environment is created by cariogenic bacteria. This study was aimed to measure pH of carious lesions intraorally using a micro-pH sensor, and assess predominant acid-producing cariogens by qPCR to differentiate caries activities. Methods 103 dentine lesions classified as active or arrested caries based on the clinical and radiological examinations were collected from patients after intraoral measurement of the lesion surface pH using a micro-pH sensor. Quantitative detection of genomic DNA copies of target cariogenic bacteria (mutans streptococci and Lactobacillus spp.) in each lesion was performed using real-time PCR. Correlation between the pH ranges and the number of bacterial species was examined by Spearman test. Results 50 samples were diagnosed as active and 53 as arrested lesions. Statistically significant difference was observed on average surface pH value between active and arrested lesions ( p < 0.05). Prevalence of Lactobacillus spp. was higher in active lesions than in arrested lesions (76% vs. 58% of samples, respectively). When the carious lesions were categorised into four different pH ranges (up to 5.5, from 5.6 to 5.8, from 5.9 to 6.1 and 6.2 or above), increased prevalence of Lactobacillus spp. was observed with decrease of pH levels. A significant negative relationship was found between pH value and number of Lactobacillus spp. ( r = −0.209, p < 0.05) but no such correlation was found for mutans streptococci. Conclusions Intraoral pH measurement might be clinically useful to determine acidity of the local environment of carious lesions as one aspect of the caries activity assessment. Clinical significance The population of certain bacteria may indicate activity of carious lesions. Intraoral pH measurement of the carious lesions using a micro-pH sensor may be a clinically feasible method for assessment of lesion acidity.
Editor: England: Elsevier Ltd
Idioma: Inglês;Persa

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Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity

Kuribayashi, Megumi ; Kitasako, Yuichi ; Matin, Khairul ; Sadr, Alireza ; Shida, Kanako ; Tagami, Junji

England: Elsevier Ltd

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