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DNA barcoding and TLC as tools to properly identify natural populations of the Mexican medicinal species Galphimia glauca Cav

Gesto-Borroto, Reinier ; Cardoso-Taketa, Alexandre ; Yactayo-Chang, Jessica P ; Medina-Jiménez, Karina ; Hornung-Leoni, Claudia ; Lorence, Argelia ; Villarreal, Maria Luisa Šiler, Branislav T.

PloS one, 2019-05, Vol.14 (5), p.e0217313-e0217313 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
DNA barcoding and TLC as tools to properly identify natural populations of the Mexican medicinal species Galphimia glauca Cav
Autor: Gesto-Borroto, Reinier ; Cardoso-Taketa, Alexandre ; Yactayo-Chang, Jessica P ; Medina-Jiménez, Karina ; Hornung-Leoni, Claudia ; Lorence, Argelia ; Villarreal, Maria Luisa
Šiler, Branislav T.
Assuntos: Anesthesia ; Animal behavior ; Animals ; Anti-Anxiety Agents - isolation & purification ; Antianxiety agents ; Bar codes ; Base Sequence ; Bioactive compounds ; Biology and Life Sciences ; Central nervous system ; Chromatography ; Chromatography, Thin Layer ; Computer and Information Sciences ; Deoxyribonucleic acid ; DNA ; DNA Barcoding, Taxonomic ; DNA, Plant - genetics ; Endemic plants ; Galphimia ; Galphimia - chemistry ; Galphimia - classification ; Galphimia - genetics ; Gene sequencing ; Genes, Plant ; Genetic testing ; Genomics ; Genotypes ; Herbal medicine ; Hispanic Americans ; Humans ; Hypnotics and Sedatives - isolation & purification ; Illnesses ; Inflammation ; Materia medica, Vegetable ; Medical research ; Medicinal plants ; Metabolism ; Methods ; Mexico ; Mice ; Natural populations ; Nervous system diseases ; Organic chemistry ; People and places ; Phenotypes ; Phylogenetics ; Phylogeny ; Plant extracts ; Plant genetics ; Plants, Medicinal - chemistry ; Plants, Medicinal - classification ; Plants, Medicinal - genetics ; Population studies ; Populations ; Quality control ; Research and analysis methods ; Species ; Species Specificity ; Studies ; Thin layer chromatography ; Tranquilizing agents
É parte de: PloS one, 2019-05, Vol.14 (5), p.e0217313-e0217313
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Galphimia glauca is a plant that is endemic to Mexico and has been commonly used since pre-Hispanic times to treat various illnesses, including central nervous system disorders and inflammation. The first studies investigating a natural population of G. glauca in Mexico showed that the plant has anxiolytic and sedative activities in mice and humans. The plant's bioactive compounds were isolated and identified, and they belong to a family of nor-secofriedelanes called galphimines. The integration of DNA barcoding and thin-layer chromatography analysis was performed to clarify whether the botanical classification of the populations in the study, which were collected in different regions of Mexico, as G. glauca was correct or if the populations consist of more than one species of the genus Galphimia. We employed six DNA barcodes (matK, rbcL, rpoC1, psbA-trnH, ITS1 and ITS2) that were analyzed individually and in combination and then compared each other, to indicate differences among the studied populations. In the phylogenetic analysis, ITS1 and ITS2 markers as well as the combination of all DNA regions were the most efficient for discriminating the population studied. The thin-layer chromatography analysis exhibited four principal chemical profiles, one of which corresponded to the populations that produced galphimines. DNA barcoding was consistent and enabled us to differentiate the populations that produce galphimines from those that do not. The results of this investigation suggest that the studied populations belong to at least four different species of the genus Galphimia. The phylogenetic analysis and the thin-layer chromatography chemical profiles were convenient tools for establishing a strong relationship between the genotype and phenotype of the studied populations and could be used for quality control purposes to prepare herbal medicines from plants of the genus Galphimia.
Editor: United States: Public Library of Science
Idioma: Inglês

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DNA barcoding and TLC as tools to properly identify natural populations of the Mexican medicinal species Galphimia glauca Cav

Gesto-Borroto, Reinier ; Cardoso-Taketa, Alexandre ; Yactayo-Chang, Jessica P ; Medina-Jiménez, Karina ; Hornung-Leoni, Claudia ; Lorence, Argelia ; Villarreal, Maria Luisa Šiler, Branislav T.

United States: Public Library of Science

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