Idioma:

Production and scavenging of nitric oxide by barley [Hordeum vulgare] root mitochondria

Gupta, K.J., University of Wuerzburg (Germany). Julius-von-Sachs Inst. for Biosciences ; Kaiser, W.M

Plant and cell physiology, 2010-04, Vol.51 (4), p.576-584 [Periódico revisado por pares]

Japan: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
Production and scavenging of nitric oxide by barley [Hordeum vulgare] root mitochondria
Autor: Gupta, K.J., University of Wuerzburg (Germany). Julius-von-Sachs Inst. for Biosciences ; Kaiser, W.M
Assuntos: Chemiluminescence ; FLUORESCENCE ; FLUORESCENCIA ; Hordeum - metabolism ; HORDEUM VULGARE ; http://www.fao.org/aos/agrovoc#c_10935 ; http://www.fao.org/aos/agrovoc#c_3662 ; http://www.fao.org/aos/agrovoc#c_36996 ; http://www.fao.org/aos/agrovoc#c_4869 ; http://www.fao.org/aos/agrovoc#c_6651 ; Luminescent Measurements ; MITOCHONDRIA ; Mitochondria - metabolism ; MITOCHONDRIE ; MITOCONDRIA ; NAD - metabolism ; NITRIC OXIDE ; Nitric Oxide - metabolism ; Nitric oxide synthase ; Nitric Oxide Synthase - metabolism ; Nitrite ; Nitrites - metabolism ; OXIDO NITRICO ; OXYDE NITRIQUE ; Plant Roots - metabolism ; RACINE ; RAICES ; ROOTS
É parte de: Plant and cell physiology, 2010-04, Vol.51 (4), p.576-584
Notas: F60
2010003527
ark:/67375/HXZ-2CCMWR00-B
ArticleID:pcq022
istex:5228C72D190075E76D3CD04A5AC8BB73AA6E60C0
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: We examined whether root mitochondria and mitochondrial membranes produce nitric oxide (NO) exclusively by reduction of nitrite or also via a nitric oxide synthase (NOS), and to what extent direct NO measurements could become undetectable due to NO oxidation. Chemiluminescence detection of NO in the gas phase was used to monitor NO emission from suspensions (i.e. direct chemiluminescence). For comparison, diaminofluorescein (DAF) and diaminorhodamine (DAR) were used as NO indicators. NO oxidation to nitrite and nitrate was quantified after reduction of nitrite + nitrate to NO by vandium (III) with subsequent chemiluminescence detection (i.e. indirect chemiluminescence). Nitrite and NADH consumption were also measured. Anaerobic nitrite-dependent NO emission was exclusively associated with the membrane fraction, without participation of matrix components. Rates of nitrite and NADH consumption matched, whereas the rate of NO emission was lower. In air, mitochondria apparently produced no nitrite-dependent NO, and no NOS activity was detected by direct or indirect chemiluminescence. In contrast, with DAF-2 or DAR-4M, an L-arginine-dependent fluorescence increase took place. However, the response of this apparent low NOS activity to inhibitors, substrates and cofactors was untypical when compared with commercial inducible NOS (iNOS), and the existence of NOS in root mitochondria is therefore doubtful. In a solution of commercial iNOS, about two-thirds of the NO (measured as NADPH consumption) were oxidized to nitrite + nitrate. Addition of mitochondria to iNOS decreased the apparent NO emission, but without a concomitant increase in nitrite + nitrate formation. Thus, mitochondria appeared to accelerate oxidation of NO to volatile intermediates.
Editor: Japan: Oxford University Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Production and scavenging of nitric oxide by barley [Hordeum vulgare] root mitochondria

Gupta, K.J., University of Wuerzburg (Germany). Julius-von-Sachs Inst. for Biosciences ; Kaiser, W.M

Japan: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.