Idioma:

Fructose 1-6 Bisphosphate Versus University of Wisconsin Solution for Rat Liver Preservation: Does FBP Prevent Early Mitochondrial Injury?

de Fraga, R.S ; Heinen, P.E.T ; Kruel, C.R.P ; Molin, S.D ; Mota, S.M ; Cerski, C.T.S ; Gasperin, G ; Souto, A.A ; de Oliveira, J.R ; Álvares-da-Silva, M.R

Transplantation proceedings, 2011-06, Vol.43 (5), p.1468-1473 [Periódico revisado por pares]

Amsterdam: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Fructose 1-6 Bisphosphate Versus University of Wisconsin Solution for Rat Liver Preservation: Does FBP Prevent Early Mitochondrial Injury?
Autor: de Fraga, R.S ; Heinen, P.E.T ; Kruel, C.R.P ; Molin, S.D ; Mota, S.M ; Cerski, C.T.S ; Gasperin, G ; Souto, A.A ; de Oliveira, J.R ; Álvares-da-Silva, M.R
Assuntos: Animals ; Biological and medical sciences ; Fructosediphosphates - administration & dosage ; Fructosediphosphates - pharmacology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Liver ; Male ; Medical sciences ; Mitochondria, Liver - drug effects ; Mitochondria, Liver - pathology ; Preservation, Biological ; Prevention and actions ; Public health. Hygiene ; Public health. Hygiene-occupational medicine ; Rats ; Rats, Wistar ; Solutions ; Surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tissue, organ and graft immunology
É parte de: Transplantation proceedings, 2011-06, Vol.43 (5), p.1468-1473
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Abstract Background Fructose 1,6-biphosphate (FBP) has been shown to exert therapeutic effects in models of ischemia-reperfusion in organs other than the liver. This study compared FBP and University of Wisconsin (UW) solution during cold storage and reperfusion, among mitochondria of adult male Wistar rat livers. Methods Adult male Wistar rats were assigned to two groups according to the preservation solution used; UW or FBP Aspartate transaminase (AST), alanine transferase (ALT); and lactic dehydrogenase (LDH) were measured in samples of the storage solution obtained at 2, 4 and 6 hours of preservation. After 6 hours of cold storage, we reperfused the liver, taking blood samples to measure AST, ALT, LDH, and throbarbituric acid reactive substances (TBARS). Hepatic fragments were processed for histologic analysis; for determinations of TBARS, catalase, and nitric oxide as well as for mitochondrial evaluation by infrared spectroscopy. Results During cold preservation, levels of AST and LDH in the storage solution were lower among the FBP group, but after reperfusion, serum levels of AST, ALT, and LDH were higher in this group, as was catalase activity. TBARS and nitric oxide were comparable between the groups. In the UW group there was a higher amide I/amide II ratio than in the FBP group, suggesting an abnormal protein structure of the mitochondrial membrane. No signs of preservation injury were observed in any liver biopsy, but sinusoidal congestion was present in livers preserved with FBP. Conclusion FBP showed a protective effect for preservation during cold storage seeming to protect the mitochondrial membrane although it did not prevent reperfusion injury.
Editor: Amsterdam: Elsevier Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 14 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Fructose 1-6 Bisphosphate Versus University of Wisconsin Solution for Rat Liver Preservation: Does FBP Prevent Early Mitochondrial Injury?

de Fraga, R.S ; Heinen, P.E.T ; Kruel, C.R.P ; Molin, S.D ; Mota, S.M ; Cerski, C.T.S ; Gasperin, G ; Souto, A.A ; de Oliveira, J.R ; Álvares-da-Silva, M.R

Amsterdam: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.