Idioma:

Lassa and mopeia virus replication in human monocytes/macrophages and in endothelial cells: Different effects on IL-8 and TNF-α gene expression

Lukashevich, Igor S. ; Maryankova, Raisa ; Vladyko, Alexander S. ; Nashkevich, Natalia ; Koleda, Svetlana ; Djavani, Mahmoud ; Horejsh, Douglas ; Voitenok, Nikolai N. ; Salvato, Maria S.

Journal of medical virology, 1999-12, Vol.59 (4), p.552-560 [Periódico revisado por pares]

New York: John Wiley & Sons, Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Lassa and mopeia virus replication in human monocytes/macrophages and in endothelial cells: Different effects on IL-8 and TNF-α gene expression
Autor: Lukashevich, Igor S. ; Maryankova, Raisa ; Vladyko, Alexander S. ; Nashkevich, Natalia ; Koleda, Svetlana ; Djavani, Mahmoud ; Horejsh, Douglas ; Voitenok, Nikolai N. ; Salvato, Maria S.
Assuntos: Arenaviridae - physiology ; Arenaviridae Infections - immunology ; Arenaviridae Infections - virology ; Cells, Cultured ; Endothelium, Vascular - virology ; Humans ; IL-8 ; inflammatory cytokines ; Interleukin-8 - genetics ; Interleukin-8 - metabolism ; Lassa Fever - immunology ; Lassa Fever - virology ; Lassa virus ; Lassa virus - physiology ; Lipopolysaccharides - pharmacology ; Macrophages - virology ; Monocytes - physiology ; Monocytes - virology ; RNA, Messenger - metabolism ; TNF-α ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; Umbilical Veins ; viral hemorrhagic fevers ; Virus Replication
É parte de: Journal of medical virology, 1999-12, Vol.59 (4), p.552-560
Notas: istex:CA9AD81A3ECCA91CFCAD76C7566335024465ECCA
NIH (to MS) - No. Ro1-AI32107
ark:/67375/WNG-RR6N1DKF-R
ArticleID:JMV21
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Cells of the mononuclear and endothelial lineages are targets for viruses which cause hemorrhagic fevers (HF) such as the filoviruses Marburg and Ebola, and the arenaviruses Lassa and Junin. A recent model of Marburg HF pathogenesis proposes that virus directly causes endothelial cell damage and macrophage release of TNF‐α which increases the permeability of endothelial monolayers [Feldmann et al., 1996]. We show that Lassa virus replicates in human monocytes/macrophages and endothelial cells without damaging them. Human endothelial cells (HUVEC) are highly susceptible to infection by both Lassa and Mopeia (a non‐pathogenic Lassa‐related arenavirus). Whereas monocytes must differentiate into macrophages before supporting even low level production of these viruses, the virus yields in the culture medium of infected HUVEC cells reach more than 7 log10 PFU/ml without cellular damage. In contrast to filovirus, Lassa virus replication in monocytes/macrophages fails to stimulate TNF‐α gene expression and even down‐regulates LPS‐stimulated TNF‐α mRNA synthesis. The expression of IL‐8, a prototypic proinflammatory CXC chemokine, was also suppressed in Lassa virus infected monocytes/macrophages and HUVEC on both the protein and mRNA levels. This contrasts with Mopeia virus infection of HUVEC in which neither IL‐8 mRNA nor protein are reduced. The cumulative down‐regulation of TNF‐α and IL‐8 expression could explain the absence of inflammatory and effective immune responses in severe cases of Lassa HF. J. Med. Virol. 59:552–560, 1999. © 1999 Wiley‐Liss, Inc.
Editor: New York: John Wiley & Sons, Inc
Idioma: Inglês;Russo

Links

Voltar para lista de resultados

Anterior Resultado 18 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Lassa and mopeia virus replication in human monocytes/macrophages and in endothelial cells: Different effects on IL-8 and TNF-α gene expression

Lukashevich, Igor S. ; Maryankova, Raisa ; Vladyko, Alexander S. ; Nashkevich, Natalia ; Koleda, Svetlana ; Djavani, Mahmoud ; Horejsh, Douglas ; Voitenok, Nikolai N. ; Salvato, Maria S.

New York: John Wiley & Sons, Inc

Buscando em bases de dados remotas. Favor aguardar.