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In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template

Abbah, Sunny-Akogwu ; Liu, Jing ; Lam, Raymond W.M. ; Goh, James C.H. ; Wong, Hee-Kit

Journal of controlled release, 2012-09, Vol.162 (2), p.364-372 [Periódico revisado por pares]

Kidlington: Elsevier B.V

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Título:
In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template
Autor: Abbah, Sunny-Akogwu ; Liu, Jing ; Lam, Raymond W.M. ; Goh, James C.H. ; Wong, Hee-Kit
Assuntos: acids ; Alginates - chemistry ; Alkaline Phosphatase - metabolism ; animal models ; Animals ; anions ; bioactive properties ; Biological and medical sciences ; biomechanics ; bone formation ; Bone Morphogenetic Protein 2 - administration & dosage ; Bone Morphogenetic Protein 2 - chemistry ; bone morphogenetic proteins ; cations ; Cell Line ; Cell Survival - drug effects ; Controlled-release ; Core-shell structure ; DEAE-Dextran - administration & dosage ; DEAE-Dextran - chemistry ; Drug Carriers - administration & dosage ; Drug Carriers - chemistry ; electrolytes ; electrostatic interactions ; General pharmacology ; Glucuronic Acid - chemistry ; heparin ; Heparin - administration & dosage ; Heparin - chemistry ; Hexuronic Acids - chemistry ; humans ; Male ; Medical sciences ; Mice ; Microspheres ; Osteogenesis - drug effects ; Peptides - administration & dosage ; Peptides - chemistry ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Polyelectrolyte complexes ; Rats ; Rats, Sprague-Dawley ; Recombinant human bone morphogenetic protein-2 (rhBMP-2) ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - chemistry ; Spinal fusion ; Spine ; tissue engineering ; Transforming Growth Factor beta - administration & dosage ; Transforming Growth Factor beta - chemistry
É parte de: Journal of controlled release, 2012-09, Vol.162 (2), p.364-372
Notas: http://dx.doi.org/10.1016/j.jconrel.2012.07.027
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Descrição: Electrostatic interactions between polycations and polyanions are being explored to fabricate polyelectrolyte complexes (PEC) that could entrap and regulate the release of a wide range of biomolecules. Here, we report the in vivo application of PEC shells fabricated from three different polycations: poly-l-ornithine (PLO), poly-l-arginine (PLA) and DEAE-dextran (DEAE-D) to condense heparin on the surface of alginate microbeads and further control the delivery of recombinant human bone morphogenetic protein 2 (rhBMP-2) in spinal fusion application. We observed large differences in the behavior of PEC shells fabricated from the cationic polyamino acids (PLO and PLA) when compared to the cationic polysaccharide, DEAE-D. Whereas DEAE-D-based PEC shells eroded and released rhBMP-2 over 2days in vitro, PLO- and PLA-based shells retained at least 60% of loaded rhBMP-2 after 3weeks of incubation in phosphate-buffered saline. In vivo implantation in a rat model of posterolateral spinal fusion revealed robust bone formation in the PLO- and PLA-based PEC shell groups. This resulted in a significantly enhanced mechanical stability of the fused segments. However, bone induction and biomechanical stability of spine segments implanted with DEAE-D-based carriers were significantly inferior to both PLO- and PLA-based PEC shell groups (p<0.01). From these results, we conclude that PEC shells incorporating native heparin could be used for growth factor delivery in functional bone tissue engineering application and that PLA- and PLO-based complexes could represent superior options to DEAE-D for loading and in vivo delivery of bioactive BMP-2 in this approach. [Display omitted]
Editor: Kidlington: Elsevier B.V
Idioma: Inglês

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In vivo bioactivity of rhBMP-2 delivered with novel polyelectrolyte complexation shells assembled on an alginate microbead core template

Abbah, Sunny-Akogwu ; Liu, Jing ; Lam, Raymond W.M. ; Goh, James C.H. ; Wong, Hee-Kit

Kidlington: Elsevier B.V

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