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Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression

Regatieri, C V ; Dreyfuss, J L ; Melo, G B ; Lavinsky, D ; Hossaka, S K ; Rodrigues, E B ; Farah, M E ; Maia, M ; Nader, H B

Brazilian journal of medical and biological research, 2010-07, Vol.43 (7), p.627-633 [Periódico revisado por pares]

Brazil: Associação Brasileira de Divulgação Científica

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Título:
Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression
Autor: Regatieri, C V ; Dreyfuss, J L ; Melo, G B ; Lavinsky, D ; Hossaka, S K ; Rodrigues, E B ; Farah, M E ; Maia, M ; Nader, H B
Assuntos: Angiogenesis ; Animals ; BIOLOGY ; Choroidal neovascularization ; Choroidal Neovascularization - etiology ; Choroidal Neovascularization - metabolism ; Choroidal Neovascularization - pathology ; Confocal scanning laser ophthalmoscope ; Female ; Fluorescein angiogram ; Fluorescein Angiography - methods ; Heparan Sulfate Proteoglycans - analysis ; Heparan Sulfate Proteoglycans - metabolism ; Immunohistochemistry ; Laser Coagulation ; MEDICINE, RESEARCH & EXPERIMENTAL ; Ophthalmoscopy - methods ; Proteoglycans ; Rats ; Rats, Zucker ; Reverse Transcriptase Polymerase Chain Reaction ; Syndecan-4 - analysis ; Syndecan-4 - metabolism
É parte de: Brazilian journal of medical and biological research, 2010-07, Vol.43 (7), p.627-633
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The objective of the present study was to develop a quantitative method to evaluate laser-induced choroidal neovascularization (CNV) in a rat model using Heidelberg Retina Angiograph 2 (HRA2) imaging. The expression of two heparan sulfate proteoglycans (HSPG) related to inflammation and angiogenesis was also investigated. CNV lesions were induced with argon laser in 21 heterozygous Zucker rats and after three weeks a fluorescein angiogram and autofluorescence exams were performed using HRA2. The area and greatest linear dimension were measured by two observers not aware of the protocol. Bland-Altman plots showed agreement between the observers, suggesting that the technique was reproducible. After fluorescein angiogram, HSPG (perlecan and syndecan-4) were analyzed by real-time RT-PCR and immunohistochemistry. There was a significant increase in the expression of perlecan and syndecan-4 (P < 0.0001) in retinas bearing CNV lesions compared to control retinas. The expression of these two HSPG increased with increasing CNV area. Immunohistochemistry demonstrated that the rat retina damaged with laser shots presented increased expression of perlecan and syndecan-4. Moreover, we observed that the overexpression occurred in the outer layer of the retina, which is related to choroidal damage. It was possible to develop a standardized quantitative method to evaluate CNV in a rat model using HRA2. In addition, we presented data indicating that the expression of HSPG parallels the area of CNV lesion. The understanding of these events offers opportunities for studies of new therapeutic interventions targeting these HSPG.
Editor: Brazil: Associação Brasileira de Divulgação Científica
Idioma: Inglês;Português

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Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression

Regatieri, C V ; Dreyfuss, J L ; Melo, G B ; Lavinsky, D ; Hossaka, S K ; Rodrigues, E B ; Farah, M E ; Maia, M ; Nader, H B

Brazil: Associação Brasileira de Divulgação Científica

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