Idioma:

Characterization of a Calcium-Activated Chloride Channel as a Shared Target of Th2 Cytokine Pathways and Its Potential Involvement in Asthma

Zhou, Yuhong ; Dong, Qu ; Louahed, Jamila ; Dragwa, Carl ; Savio, Dawn ; Huang, Minxue ; Weiss, Christine ; Tomer, Yaniv ; McLane, Michael P ; Nicolaides, Nicholas C ; Levitt, Roy C

American journal of respiratory cell and molecular biology, 2001-10, Vol.25 (4), p.486-491 [Periódico revisado por pares]

United States: Am Thoracic Soc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Characterization of a Calcium-Activated Chloride Channel as a Shared Target of Th2 Cytokine Pathways and Its Potential Involvement in Asthma
Autor: Zhou, Yuhong ; Dong, Qu ; Louahed, Jamila ; Dragwa, Carl ; Savio, Dawn ; Huang, Minxue ; Weiss, Christine ; Tomer, Yaniv ; McLane, Michael P ; Nicolaides, Nicholas C ; Levitt, Roy C
Assuntos: Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - pharmacology ; Asthma - metabolism ; Chloride Channels - genetics ; Chloride Channels - metabolism ; Cloning, Molecular ; Cytokines - metabolism ; Humans ; In Situ Hybridization - methods ; Interleukin-9 - genetics ; Interleukin-9 - immunology ; Interleukin-9 - metabolism ; Lung - physiology ; Mice ; Mice, Inbred Strains ; Mice, Transgenic ; Molecular Sequence Data ; Mucoproteins - genetics ; Mucoproteins - metabolism ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Respiratory Mucosa - physiology ; Signal Transduction ; Th2 Cells - metabolism
É parte de: American journal of respiratory cell and molecular biology, 2001-10, Vol.25 (4), p.486-491
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Interleukin (IL)-9 is a T helper (Th) 2 cytokine recently implicated as an essential factor in determining susceptibility to asthma. Transgenic mice overexpressing IL-9 exhibit many features that are characteristic of human asthma. To better understand the mechanism by which IL-9 mediates the various biologic activities in asthma, we performed suppressive subtraction hybridization with whole lung from IL-9 transgenic and control mice. Here we report the identification of mCLCA3, a calcium-activated chloride channel that was specifically induced in the lung epithelium of IL-9 transgenic mice. Expression of mCLCA3 could also be induced by intratracheal administration of IL-9 or other Th2 cytokines (IL-4, IL-13), but not by interferon-gamma. Moreover, expression of mCLCA3 was induced in the lung of antigen-exposed mice, and this induction could be suppressed by neutralizing IL-9 antibody treatment, indicating IL-9 is both necessary and sufficient to induce mCLCA3 in this experimental model of asthma. Finally, we demonstrate that hCLCA1 is the human counterpart to mCLCA3 and is also induced in vitro in human primary lung cells by Th2 cytokine treatment. Together, these data strongly implicate the involvement of mCLCA3 (in mice) and hCLCA1 (in humans) in the pathogenesis of Th2 cytokine-mediated asthmatic disorders.
Editor: United States: Am Thoracic Soc
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 8 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Characterization of a Calcium-Activated Chloride Channel as a Shared Target of Th2 Cytokine Pathways and Its Potential Involvement in Asthma

Zhou, Yuhong ; Dong, Qu ; Louahed, Jamila ; Dragwa, Carl ; Savio, Dawn ; Huang, Minxue ; Weiss, Christine ; Tomer, Yaniv ; McLane, Michael P ; Nicolaides, Nicholas C ; Levitt, Roy C

United States: Am Thoracic Soc

Buscando em bases de dados remotas. Favor aguardar.