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EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

Kalina, T ; Flores-Montero, J ; van der Velden, V H J ; Martin-Ayuso, M ; Böttcher, S ; Ritgen, M ; Almeida, J ; Lhermitte, L ; Asnafi, V ; Mendonça, A ; de Tute, R ; Cullen, M ; Sedek, L ; Vidriales, M B ; Pérez, J J ; te Marvelde, J G ; Mejstrikova, E ; Hrusak, O ; Szczepański, T ; van Dongen, J J M ; Orfao, A

Leukemia, 2012-09, Vol.26 (9), p.1986-2010 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
Autor: Kalina, T ; Flores-Montero, J ; van der Velden, V H J ; Martin-Ayuso, M ; Böttcher, S ; Ritgen, M ; Almeida, J ; Lhermitte, L ; Asnafi, V ; Mendonça, A ; de Tute, R ; Cullen, M ; Sedek, L ; Vidriales, M B ; Pérez, J J ; te Marvelde, J G ; Mejstrikova, E ; Hrusak, O ; Szczepański, T ; van Dongen, J J M ; Orfao, A
Assuntos: Antibodies ; Antibodies, Monoclonal ; Biomarkers, Tumor - immunology ; Diagnosis ; Europe ; Flow cytometry ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Flow Cytometry - standards ; Hematologic Neoplasms - diagnosis ; Hematologic Neoplasms - immunology ; Hematology ; Human health and pathology ; Humans ; Immunophenotyping - standards ; Laboratories - standards ; Leukemia ; Life Sciences ; Physiological aspects ; Prognosis ; Special Report ; Viral antibodies
É parte de: Leukemia, 2012-09, Vol.26 (9), p.1986-2010
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
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PMCID: PMC3437409
Shared first authorship, because TK and JFM have equally contributed to this manuscript.
Descrição: The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
Editor: England: Nature Publishing Group
Idioma: Inglês

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EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

England: Nature Publishing Group

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