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Reverse chemical genetics methods and protocols
Hisashi Koga
New York, NY Humana Press c2009
Localização:
CQ - Conjunto das Químicas
(574.873224 R452 )
(Acessar)
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Título:
Reverse chemical genetics methods and protocols
Autor:
Hisashi Koga
Assuntos:
Biochemical genetics
;
GENÉTICA BIOQUÍMICA
;
BIOLOGIA MOLECULAR
;
Combinatorial Chemistry Techniques -- methods -- Laboratory Manuals
;
Genetic Techniques -- Laboratory Manuals
;
Molecular Biology -- methods -- Laboratory Manuals
Notas:
Includes bibliographical references and index.
Descrição:
ORFeome cloning / Osamu Ohara -- Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida -- High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa -- Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono -- High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi ... [et al.] -- Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey -- High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga -- An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki ... [et al.] -- Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya -- Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi ... [et al.] -- Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi -- Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama -- Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara -- Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka -- Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu ... [et al.] -- Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya ... [et al.] -- High-throughput SPR biosensor / Motoki Kyo ... [et al.] -- Drug discovery through functional screening in the Drosophi
Títulos relacionados:
Série:Springer protocols; Methods in molecular biology (Clifton, N.J.) v. 577
Editor:
New York, NY Humana Press
Data de criação/publicação:
c2009
Formato:
xi, 272 p ill. (some col.) 27 cm.
Idioma:
Inglês
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