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Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil

T A R Caruzo V Munford; W M E D Brito; S S Kroefs; Edison Luiz Durigon; Maria Lucia Barbosa de Oliveira Racz; Congresso Instituto Ciências Biomédicas, IV (2002 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2002

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  • Título:
    Sequence analysis of an atypical P[8] bovine rotavirus sample from state of Goiás, Brazil
  • Autor: T A R Caruzo
  • V Munford; W M E D Brito; S S Kroefs; Edison Luiz Durigon; Maria Lucia Barbosa de Oliveira Racz; Congresso Instituto Ciências Biomédicas, IV (2002 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2002
  • Descrição: Rotaviruses are the major etiologic agents of acute dehydrating diarrhea in many mammalian species, including calves. Members of the family Reoviridae, they have a genome consisting of 11 segments of dsRNA, each one coding for at least one protein. Two structural proteins located in the outer capsid (VP4 and VP7) are used for molecular characterization of G and P genotypes by RT-PCR and by genome sequencing. BoRV-A sample ICB896, characterized as G3Pnegative by RT-PCR using specific primers for animal and human genotypes, had its VP4 gene partially sequenced in an ABI-Prism 377 DNA Sequencer, with Big Dye kit. Nucleotide sequence analysis showed that ICB896 had a homology of 90,1% with P[8]HuRV-A/WA and that at the P[8]-specific primer binding position (339-356) 5 mismatches were present, which could have prevented primer 1T.1 from binding to ICB896 during RT-PCR. These results suggest that molecular characterization of BoRV-A by RT-PCR can lead to non-characterized genotypes due to differences in the nucleotide sequence of the primer-binding site, and that gene sequencing then become important for more precise characterizations. Also, a BoRV-A sample characterized as a HuRV-A P genotype may suggest inter-species transmission of this virus. This finding may contribute for the development of more efficient and specific bovine rotavirus vaccines worldwide
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2002
  • Formato: 1. (várias paginações) poster 382.; Disponível em CD-ROM.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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