skip to main content
Idioma:

High nitrogen concentration causes G2/M arrest in Hanseniaspora vineae

Schwarz, Luisa Vivian ; Sandri, Fernanda Knaach ; Scariot, Fernando ; Delamare, Ana Paula Longaray ; Valera, Maria Jose ; Carrau, Francisco ; Echeverrigaray, Sergio

Yeast (Chichester, England), 2023-12, Vol.40 (12), p.640-650 [Periódico revisado por pares]

England: Wiley Subscription Services, Inc

Texto completo disponível

Citações Citado por
  • Título:
    High nitrogen concentration causes G2/M arrest in Hanseniaspora vineae
  • Autor: Schwarz, Luisa Vivian ; Sandri, Fernanda Knaach ; Scariot, Fernando ; Delamare, Ana Paula Longaray ; Valera, Maria Jose ; Carrau, Francisco ; Echeverrigaray, Sergio
  • Assuntos: Acidification ; Ammonium ; Apoptosis ; Cell cycle ; Cell Line, Tumor ; DNA damage ; DNA fragmentation ; DNA repair ; G2 Phase Cell Cycle Checkpoints ; G2/M arrest ; Genetic factors ; Hanseniaspora - metabolism ; Hanseniaspora vineae ; Hyperpolarization ; Intracellular ; Nitrogen ; Nitrogen - metabolism ; quiescense ; Reactive oxygen species ; Stationary phase ; yeasts
  • É parte de: Yeast (Chichester, England), 2023-12, Vol.40 (12), p.640-650
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Yeasts have been widely used as a model to better understand cell cycle mechanisms and how nutritional and genetic factors can impact cell cycle progression. While nitrogen scarcity is well known to modulate cell cycle progression, the relevance of nitrogen excess for microorganisms has been overlooked. In our previous work, we observed an absence of proper entry into the quiescent state in Hanseniaspora vineae and identified a potential link between this behavior and nitrogen availability. Furthermore, the Hanseniaspora genus has gained attention due to a significant loss of genes associated with DNA repair and cell cycle. Thus, the aim of our study was to investigate the effects of varying nitrogen concentrations on H. vineae's cell cycle progression. Our findings demonstrated that nitrogen excess, regardless of the source, disrupts cell cycle progression and induces G2/M arrest in H. vineae after reaching the stationary phase. Additionally, we observed a viability decline in H. vineae cells in an ammonium‐dependent manner, accompanied by increased production of reactive oxygen species, mitochondrial hyperpolarization, intracellular acidification, and DNA fragmentation. Overall, our study highlights the events of the cell cycle arrest in H. vineae induced by nitrogen excess and attempts to elucidate the possible mechanism triggering this absence of proper entry into the quiescent state. Nitrogen excess induces G2/M arrest in Hanseniaspora vineae after the stationary phase, a reduction in cell viability, an increase in reactive oxygen species production, mitochondrial hyperpolarization, intracellular acidification, and DNA damage. Take‐away High concentrations of nitrogen inhibit proper quiescent arrest in Hanseniaspora vineae. The decline in viability of yeast is ammonium‐dependent. Ammonium excess leads to an increase in reactive oxygen species and mitochondrial hyperpolarization. Ammonium excess triggers intracellular acidification and DNA damage.
  • Editor: England: Wiley Subscription Services, Inc
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.