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Fed-Batch Process for Pyruvate Production by Recombinant Escherichia coli YYC202 Strain

Zelić, B. ; Gerharz, T. ; Bott, M. ; Vasić-Rački, Đ. ; Wandrey, C. ; Takors, R.

Engineering in life sciences, 2003-07, Vol.3 (7), p.299-305 [Periódico revisado por pares]

Weinheim: WILEY-VCH Verlag

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  • Título:
    Fed-Batch Process for Pyruvate Production by Recombinant Escherichia coli YYC202 Strain
  • Autor: Zelić, B. ; Gerharz, T. ; Bott, M. ; Vasić-Rački, Đ. ; Wandrey, C. ; Takors, R.
  • Assuntos: Batch processes ; Biological and medical sciences ; Biotechnology ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Microorganisms
  • É parte de: Engineering in life sciences, 2003-07, Vol.3 (7), p.299-305
  • Notas: istex:6A30D8466A3049B073E5B548F2FC86BF93F3A025
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  • Descrição: Fed‐batch fermentation was applied to the production of pyruvate by using a recombinant Escherichia coli YYC202 strain. This strain is completely blocked in its ability to convert pyruvate into acetyl‐CoA or acetate, resulting in acetate auxotrophy during growth in glucose minimal medium. By controlling acetate and glucose feed rate, a series of lab‐scale fed‐batch experiments were performed at pH 7 and 37 °C. CO2 production rate (CTR) was used for on‐line regulation of the acetate feed rate. The correlation between CTR and acetate consumption rate (ACR) was determined experimentally. At optimal process conditions a final pyruvate concentration higher than 62 g/L, a space‐time yield of up to 42 g/L/d and pyruvate/glucose molar yield of 1.11 mol/mol were achieved. Experimental evidence was gathered that pyruvate export is active. Due to the importance of and the great demand for pyruvate, different processes for the production of pyruvic acid have been developed in the past. Among the biotechnological approaches published so far, the microbial pyruvate production based on C6 sources, like glucose, is the most promising one, however, a very low space‐time yield hampers its use for an industrial scale‐up. As an alternative approach that combines high product titers with high pyruvate/substrate yields and also with much higher space‐time yields at optimal process conditions, a fed‐batch process was developed by using a recombinant Escherichia coli YYC202 strain.
  • Editor: Weinheim: WILEY-VCH Verlag
  • Idioma: Inglês
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