skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Antiviral Evaluation of UV-4B and Interferon-Alpha Combination Regimens against Dengue Virus

Franco, Evelyn J ; Pires de Mello, Camilly P ; Brown, Ashley N

Viruses, 2021-04, Vol.13 (5), p.771 [Periódico revisado por pares]

Switzerland: MDPI AG

Texto completo disponível

Citações Citado por
  • Título:
    Antiviral Evaluation of UV-4B and Interferon-Alpha Combination Regimens against Dengue Virus
  • Autor: Franco, Evelyn J ; Pires de Mello, Camilly P ; Brown, Ashley N
  • Assuntos: antiviral ; Antiviral activity ; Antiviral agents ; Antiviral drugs ; Cell growth ; Cell lines ; Cell proliferation ; Dengue fever ; Dengue hemorrhagic fever ; DENV ; Experiments ; favipiravir ; Fever ; Guillain-Barre syndrome ; Infections ; Influenza ; interferon ; Plaque assay ; sofosbuvir ; UV-4B ; Vero cells ; Viral infections ; Viruses ; α-Interferon
  • É parte de: Viruses, 2021-04, Vol.13 (5), p.771
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    These authors contributed equally to this work.
  • Descrição: Dengue virus (DENV) is a flavivirus associated with clinical manifestations ranging in severity from self-limiting dengue fever, to the potentially life threatening condition, severe dengue. There are currently no approved antiviral therapies for the treatment of DENV. Here, we evaluated the antiviral potential of four broad-spectrum antivirals, UV-4B, interferon-alpha (IFN), sofosbuvir (SOF), and favipiravir (FAV) against DENV serotype 2 as mono- and combination therapy in cell lines that are physiologically relevant to human infection. Cell lines derived from human liver (HUH-7), neurons (SK-N-MC), and skin (HFF-1) were infected with DENV and treated with UV-4B, IFN, SOF, or FAV. Viral supernatant was sampled daily and infectious viral burden was quantified by plaque assay on Vero cells. Drug effect on cell proliferation in uninfected and infected cells was also assessed. UV-4B inhibited DENV in HUH-7, SK-N-MC, and HFF-1 cells yielding EC values of 23.75, 49.44, and 37.38 µM, respectively. Clinically achievable IFN concentrations substantially reduced viral burden in HUH-7 (EC = 102.7 IU/mL), SK-N-MC (EC = 86.59 IU/mL), and HFF-1 (EC = 163.1 IU/mL) cells. SOF potently inhibited DENV in HUH-7 cells but failed to produce the same effect in SK-N-MC and HFF-1 cells. Finally, FAV provided minimal suppression in HUH-7 and SK-N-MC cells, but was ineffective in HFF-1 cells. The two most potent anti-DENV agents, UV-4B and IFN, were also assessed in combination. UV-4B + IFN treatment enhanced antiviral activity in HUH-7, SK-N-MC, and HFF-1 cells relative to monotherapy. Our results demonstrate the antiviral potential of UV-4B and IFN against DENV in multiple physiologically relevant cell types.
  • Editor: Switzerland: MDPI AG
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  2 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.