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Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles

Paccez, Juliano D ; Nguyen, Hoang D ; Luiz, Wilson B ; Ferreira, Rita C.C ; Sbrogio-Almeida, Maria E ; Schuman, Wolfgang ; Ferreira, Luís C.S

Vaccine, 2007-06, Vol.25 (24), p.4671-4680 [Periódico revisado por pares]

Oxford: Elsevier Ltd

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Título:
Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles
Autor: Paccez, Juliano D ; Nguyen, Hoang D ; Luiz, Wilson B ; Ferreira, Rita C.C ; Sbrogio-Almeida, Maria E ; Schuman, Wolfgang ; Ferreira, Luís C.S
Assuntos: Administration, Oral ; Allergy and Immunology ; Animals ; Antibodies, Bacterial - analysis ; Antibodies, Bacterial - blood ; Antigens, Bacterial - biosynthesis ; Antigens, Bacterial - immunology ; Antigens, Bacterial - metabolism ; Antitoxins - analysis ; Antitoxins - blood ; Applied microbiology ; B. subtilis spores ; B. subtilis vaccine vectors ; Bacillus subtilis ; Bacillus subtilis - genetics ; Bacillus subtilis - immunology ; Bacterial Proteins - genetics ; Bacterial Toxins - biosynthesis ; Bacterial Toxins - immunology ; Bacterial Toxins - metabolism ; Bacterial Vaccines - administration & dosage ; Bacterial Vaccines - genetics ; Bacterial Vaccines - immunology ; Biological and medical sciences ; Disease Models, Animal ; Enterotoxins - biosynthesis ; Enterotoxins - immunology ; Enterotoxins - metabolism ; Escherichia coli - genetics ; Escherichia coli Proteins - biosynthesis ; Escherichia coli Proteins - immunology ; Escherichia coli Proteins - metabolism ; ETEC ; Female ; Fundamental and applied biological sciences. Psychology ; Immunoglobulin A - analysis ; Immunoglobulin G - blood ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Microbiology ; Mucosal immunization ; Neutralization Tests ; Oral vaccines ; Plasmids - genetics ; Poisoning - immunology ; Promoter Regions, Genetic ; Protein Sorting Signals - genetics ; Protein Subunits - biosynthesis ; Protein Subunits - immunology ; Protein Subunits - metabolism ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - immunology ; Recombinant Proteins - metabolism ; Spores, Bacterial - immunology ; Survival Analysis ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
É parte de: Vaccine, 2007-06, Vol.25 (24), p.4671-4680
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Descrição: Abstract Recombinant Bacillus subtilis strains, either in the form of spores or vegetative cells, may be employed as safe and low-cost vaccine vehicles. In this study, we studied the role of promoter sequences and antigen-sorting signals on the immunogenicity based on previously constructed B. subtilis episomal expression systems. Mice orally immunized with spores or cells encoding the B subunit of the heat labile toxin (LTB), originally expressed by some enterotoxigenic Escherichia coli (ETEC) strains, under control of the stress-inducible gsiB promoter developed higher anti-LTB serum IgG and fecal IgA responses with regard to vaccine strains transformed with plasmids encoding the antigen under control of IPTG-inducible (P spac ) or constitutive (P lepA ) promoters. Moreover, surface expression of the vaccine antigen under the control of the P gsiB promoter enhanced the immunogenicity of vegetative cells, while intracellular accumulation of LTB led to higher antibody responses in mice orally immunized with recombinant B. subtilis spores. Specific anti-LTB antibodies raised in vaccinated mice recognized and neutralized in vitro the native toxin produced by ETEC strains. Nonetheless, only mice orally immunized with recombinant B. subtilis strains, either as vegetative cells or spores, expressing intracellular LTB under the control of the gsiB promoter conferred partial protection to lethal challenges with purified LT. The present report further demonstrates that B. subtilis plasmid-based heterologous protein expression systems are adequate for antigen delivery via the oral route.
Editor: Oxford: Elsevier Ltd
Idioma: Inglês

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Evaluation of different promoter sequences and antigen sorting signals on the immunogenicity of Bacillus subtilis vaccine vehicles

Paccez, Juliano D ; Nguyen, Hoang D ; Luiz, Wilson B ; Ferreira, Rita C.C ; Sbrogio-Almeida, Maria E ; Schuman, Wolfgang ; Ferreira, Luís C.S

Oxford: Elsevier Ltd

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