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Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production

Dashwood, Michael R. ; Savage, Kay ; Dooley, Audrey ; Shi-Wen, Xu ; Abraham, David J. ; Souza, Domingos S.R.

The Annals of thoracic surgery, 2005-09, Vol.80 (3), p.939-944 [Periódico revisado por pares]

New York, NY: Elsevier Inc

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Título:
Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production
Autor: Dashwood, Michael R. ; Savage, Kay ; Dooley, Audrey ; Shi-Wen, Xu ; Abraham, David J. ; Souza, Domingos S.R.
Assuntos: Aged ; Biological and medical sciences ; Coronary Artery Bypass - methods ; Endothelium, Vascular - metabolism ; Female ; Graft Survival ; Humans ; Male ; Medical sciences ; Middle Aged ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - biosynthesis ; Saphenous Vein - injuries ; Saphenous Vein - metabolism ; Saphenous Vein - pathology ; Saphenous Vein - transplantation ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the respiratory system ; Transplants ; Vascular Patency
É parte de: The Annals of thoracic surgery, 2005-09, Vol.80 (3), p.939-944
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Although the saphenous vein is the most commonly used conduit for coronary artery bypass surgery occlusion rates are high, with more than 50% grafts failing within 10 years. Nitric oxide, a potent vasodilator, also inhibits platelet aggregation, thrombus formation and vascular smooth muscle cell proliferation, is implicated in various vascular pathologies, including graft failure. Saphenous veins were obtained from patients undergoing bypass surgery harvested by conventional methods and with minimal handling, using a “no-touch” technique. Tissue distribution and protein expression of endothelial nitric oxide synthase was compared using immunohistochemistry and Western blot analysis. Nitric oxide generation was assessed using the citrulline assay. There was injury to conventional compared with no-touch vein segments, in particular to the lumenal endothelium and tunica adventitia. This injury was accompanied by an absence of endothelial nitric oxide synthase immunostaining at regions of endothelial denudation and damaged adventitial layer of conventional veins and a significant reduction ( p < 0.05) in endothelial nitric oxide synthase protein expression. Furthermore, nitric oxide release from conventional tissue extracts was significantly ( p < 0.05) lower than no-touch vein segments. Our results show that there is a reduction in endothelial nitric oxide synthase and nitric oxide release in saphenous veins harvested by conventional surgical methods compared with those prepared atraumatically. These observations may influence graft performance.
Editor: New York, NY: Elsevier Inc
Idioma: Inglês

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Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production

Dashwood, Michael R. ; Savage, Kay ; Dooley, Audrey ; Shi-Wen, Xu ; Abraham, David J. ; Souza, Domingos S.R.

New York, NY: Elsevier Inc

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