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Single-cell RNA sequencing reveals roles of unique retinal microglia types in early diabetic retinopathy

Wang, Yan ; Yang, Xiongyi ; Zhang, Yuxi ; Hong, Libing ; Xie, Zhuohang ; Jiang, Wenmin ; Chen, Lin ; Xiong, Ke ; Yang, Siyu ; Lin, Meiping ; Guo, Xi ; Li, Qiumo ; Deng, Xiaoqing ; Lin, Yanhui ; Cao, Mingzhe ; Yi, Guoguo ; Fu, Min

Diabetology and metabolic syndrome, 2024-02, Vol.16 (1), p.49-49, Article 49 [Periódico revisado por pares]

England: BioMed Central Ltd

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Single-cell RNA sequencing reveals roles of unique retinal microglia types in early diabetic retinopathy
Autor: Wang, Yan ; Yang, Xiongyi ; Zhang, Yuxi ; Hong, Libing ; Xie, Zhuohang ; Jiang, Wenmin ; Chen, Lin ; Xiong, Ke ; Yang, Siyu ; Lin, Meiping ; Guo, Xi ; Li, Qiumo ; Deng, Xiaoqing ; Lin, Yanhui ; Cao, Mingzhe ; Yi, Guoguo ; Fu, Min
Assuntos: Analysis ; Bar codes ; Blood ; Cells ; Diabetes ; Diabetes mellitus ; Diabetic retinopathy ; Drug screening ; Egr-2 protein ; Gene expression ; Genotype & phenotype ; Glucose ; Inflammation ; Macrophages ; Medical research ; Medicine, Experimental ; Microenvironments ; Microglia ; Microvasculature ; Morphology ; Phenotypes ; Polarization ; Regulatory sequences ; Retina ; Retinal microglia ; Retinopathy ; RNA ; RNA sequencing ; Scientific equipment and supplies industry ; Single-cell RNA sequencing
É parte de: Diabetology and metabolic syndrome, 2024-02, Vol.16 (1), p.49-49, Article 49
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SourceType-Scholarly Journals-1
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Descrição: The pathophysiological mechanisms of diabetic retinopathy (DR), a blinding disease, are intricate. DR was thought to be a microvascular disease previously. However, growing studies have indicated that the retinal microglia-induced inflammation precedes microangiopathy. The binary concept of microglial M1/M2 polarization paradigms during inflammatory activation has been debated. In this study, we confirmed microglia had the most significant changes in early DR using single-cell RNA sequencing. A total of five retinal specimens were collected from donor SD rats. Changes in various cells of the retina at the early stage of DR were analyzed using single-cell sequencing technology. We defined three new microglial subtypes at cellular level, including two M1 types (Egr2 M1 and Egr2 M1) and one M2 type. We also revealed the anatomical location between these subtypes, the dynamic changes of polarization phenotypes, and the possible activation sequence and mutual activation regulatory mechanism of different cells. Furthermore, we constructed an inflammatory network involving microglia, blood-derived macrophages and other retinal nonneuronal cells. The targeted study of new disease-specific microglial subtypes can shorten the time for drug screening and clinical application, which provided insight for the early control and reversal of DR. We found that microglia show the most obvious differential expression changes in early DR and reveal the changes in microglia in a high-glucose microenvironment at the single-cell level. Our comprehensive analysis will help achieve early reversal and control the occurrence and progression of DR.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Single-cell RNA sequencing reveals roles of unique retinal microglia types in early diabetic retinopathy

Wang, Yan ; Yang, Xiongyi ; Zhang, Yuxi ; Hong, Libing ; Xie, Zhuohang ; Jiang, Wenmin ; Chen, Lin ; Xiong, Ke ; Yang, Siyu ; Lin, Meiping ; Guo, Xi ; Li, Qiumo ; Deng, Xiaoqing ; Lin, Yanhui ; Cao, Mingzhe ; Yi, Guoguo ; Fu, Min

England: BioMed Central Ltd

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