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Effects of estradiol on human dendritic cell differentiation and maturation

A P S Azevedo José Alexandre Marzagão Barbuto; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

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  • Título:
    Effects of estradiol on human dendritic cell differentiation and maturation
  • Autor: A P S Azevedo
  • José Alexandre Marzagão Barbuto; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: IMUNOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and Objectives: Dendritic cells (DCs) are the most efficient antigen presenting cells, having a distinct role as initiators and modulators of the immune response. They can be found in two maturation stages: immature DCs (iDC) are well equipped to capture antigens, and express low level of accessory molecules for T-cell activation; mature DCs (mDCs) have decreased endocytic ability but show high surface expression of T-cell-activating molecules, as MHC-II and co-stimulatory molecules. Furthermore, during maturation the expression of the chemokine receptor (CCR7) that guides migratory DCs to T-cell areas of secondary lymphoid organs is up-regulated. Many factors influence DC maturation and since estradiol (E2) receptors are expressed by DCs in lymph nodes and tumor-associated lymphoid infiltrates we decided to investigate the effects of this hormone in DC differentiation and maturation in vitro. Methods and Results: DCs were generated from peripheral blood mononuclear cells from healthy female volunteers. Monocytes were cultured in AIM-V medium, supplemented with interleukin 4 and granulocyte-macrophage colony stimulating factor. On the fifth day, cells were stimulated with TNF-a (mDCs) or not (iDCs). Cultures were also supplemented or not with E2 (1mM), on day 0 or on day 5. On the 7th day, no clear-cut differences were noticed, by flow cytometry, in the expression of most markers analyzed, but the CCR7 expression by
    HLA-DR+ cells (as measured by the geometric mean of fluorescence) was increased by E2 treatment: iDCs' expression of this marker increased from 8.3 (controls) to 11.1 (E2 on day 0) and to 14.9 (E2 on day 5). mDCs also showed increased expression of CCR7 with E2 treatment (9.4 x 14.9 x 16.3). Conclusion: Our results suggest that E2 may increase CCR-7 expression and thus increase their migration to secondary lymphoid organs.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. CI.036.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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