skip to main content
Idioma:

Modulation of nucleotide metabolism by picornaviruses

Nouwen, Lonneke V ; Breeuwsma, Martijn ; Zaal, Esther A ; van de Lest, Chris H A ; Buitendijk, Inge ; Zwaagstra, Marleen ; Balić, Pascal ; Filippov, Dmitri V ; Berkers, Celia R ; van Kuppeveld, Frank J M

2024-03

Texto completo disponível

Citações Citado por
  • Título:
    Modulation of nucleotide metabolism by picornaviruses
  • Autor: Nouwen, Lonneke V ; Breeuwsma, Martijn ; Zaal, Esther A ; van de Lest, Chris H A ; Buitendijk, Inge ; Zwaagstra, Marleen ; Balić, Pascal ; Filippov, Dmitri V ; Berkers, Celia R ; van Kuppeveld, Frank J M
  • Assuntos: Genetics ; Immunology ; Microbiology ; Molecular Biology ; Parasitology ; Virology
  • Notas: https://dspace.library.uu.nl/handle/1874/436825
    PLoS Pathogens 20(3 March), - (2024)
    1553-7366
  • Descrição: Viruses actively reprogram the metabolism of the host to ensure the availability of sufficient building blocks for virus replication and spreading. However, relatively little is known about how picornaviruses-a large family of small, non-enveloped positive-strand RNA viruses-modulate cellular metabolism for their own benefit. Here, we studied the modulation of host metabolism by coxsackievirus B3 (CVB3), a member of the enterovirus genus, and encephalomyocarditis virus (EMCV), a member of the cardiovirus genus, using steady-state as well as 13C-glucose tracing metabolomics. We demonstrate that both CVB3 and EMCV increase the levels of pyrimidine and purine metabolites and provide evidence that this increase is mediated through degradation of nucleic acids and nucleotide recycling, rather than upregulation of de novo synthesis. Finally, by integrating our metabolomics data with a previously acquired phosphoproteomics dataset of CVB3-infected cells, we identify alterations in phosphorylation status of key enzymes involved in nucleotide metabolism, providing insight into the regulation of nucleotide metabolism during infection.
  • Data de criação/publicação: 2024-03
  • Idioma: Inglês
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.