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TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C60) Primary Ions

Fletcher, John S ; Lockyer, Nicholas P ; Vaidyanathan, Seetharaman ; Vickerman, John C

Analytical chemistry (Washington), 2007-03, Vol.79 (6), p.2199-2206 [Periódico revisado por pares]

Washington, DC: American Chemical Society

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  • Título:
    TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C60) Primary Ions
  • Autor: Fletcher, John S ; Lockyer, Nicholas P ; Vaidyanathan, Seetharaman ; Vickerman, John C
  • Assuntos: Analytical chemistry ; Animals ; Chemical bonds ; Chemistry ; Exact sciences and technology ; Fullerenes - chemistry ; Imaging, Three-Dimensional - methods ; Ions ; Ions - chemistry ; Mass spectrometry ; Molecular biology ; Oocytes - chemistry ; Spectrometric and optical methods ; Spectrometry, Mass, Secondary Ion - methods ; Xenopus laevis
  • É parte de: Analytical chemistry (Washington), 2007-03, Vol.79 (6), p.2199-2206
  • Notas: istex:F54B2CB8D05F54B3042FE4F62178AED783E9C8FC
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  • Descrição: Time-of-flight secondary ion mass spectrometry (TOF-SIMS) using buckminsterfullerene (C60) as the primary ion source has the ability to generate chemical images of surfaces with high sensitivities and minimal chemical damage. We studied the application of C60 + to depth profile a biological cell surface in a controlled manner and to subsequently image the revealed subsurfaces, in order to generate three-dimensional molecular images of the biological system. Such an analytical tool not only enables the surface localization of molecular species to be mapped but also enables the biomolecular distribution as a function of depth to be investigated with minimal sample preparation/intervention. Here we demonstrate the technique with a freeze-dried Xenopus laevis oocyte, which is a single cell. A C60 + ion beam was used with computer-controlled analyses and etch cycles. Mass spectra derived from the surface revealed peaks corresponding to cholesterol (m/z 369) and other lipids at m/z 540−570 and 800−1000, in the positive ion mode, and lipid fatty acid side chains (e.g., m/z 255) in the negative ion mode. To our knowledge, this is the first demonstration of the 3D biomolecular imaging within an actual biological system using TOF-SIMS.
  • Editor: Washington, DC: American Chemical Society
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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