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Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L

Hasan, Md Soyib ; Singh, Vishal ; Islam, Shiful ; Islam, Md Sifatul ; Ahsan, Raju ; Kaundal, Amita ; Islam, Tahmina ; Ghosh, Ajit Chakrabarty, Debasis

PloS one, 2021-02, Vol.16 (2), p.e0247170 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L
Autor: Hasan, Md Soyib ; Singh, Vishal ; Islam, Shiful ; Islam, Md Sifatul ; Ahsan, Raju ; Kaundal, Amita ; Islam, Tahmina ; Ghosh, Ajit
Chakrabarty, Debasis
Assuntos: Abiotic stress ; Agriculture ; Alfalfa ; Analysis ; Beans ; Biochemistry ; Biology ; Biology and Life Sciences ; Botany ; Cold tolerance ; Dehydration ; Ecology and Environmental Sciences ; Editing ; Electronic mail ; Elongation ; Engineering and Technology ; Enzymes ; Food security ; Gene expression ; Genes ; Genetic aspects ; Genomes ; Genomics ; Glutathione ; Glutathione transferase ; Growth ; Heavy metals ; Herbicides ; Hydroquinone ; Islam ; Legumes ; Localization ; Methodology ; Mimosaceae ; Molecular biology ; Molecular weight ; Oxidative stress ; Phylogeny ; Physical Sciences ; Physiological aspects ; Physiology ; Prostaglandin E ; Proteins ; Reductases ; Research and Analysis Methods ; Reviews ; Science and technology ; Soil investigations ; Soils ; Stress (Physiology) ; Sustainable agriculture ; Technology ; Tetrachlorohydroquinone ; Translation elongation
É parte de: PloS one, 2021-02, Vol.16 (2), p.e0247170
Notas: Competing Interests: The authors have declared that no competing interests exist.
Current address: Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
Descrição: Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well as plant development and growth. The present study aimed to a comprehensive genome-wide identification and functional characterization of GST family in one of the economically important legume plants-Medicago truncatula. Here, we have identified a total of ninety-two putative MtGST genes that code for 120 proteins. All these members were classified into twelve classes based on their phylogenetic relationship and the presence of structural conserved domain/motif. Among them, 7 MtGST gene pairs were identified to have segmental duplication. Expression profiling of MtGST transcripts revealed their high level of organ/tissue-specific expression in most of the developmental stages and anatomical tissues. The transcripts of MtGSTU5, MtGSTU8, MtGSTU17, MtGSTU46, and MtGSTU47 showed significant up-regulation in response to various abiotic and biotic stresses. Moreover, transcripts of MtGSTU8, MtGSTU14, MtGSTU28, MtGSTU30, MtGSTU34, MtGSTU46 and MtGSTF8 were found to be highly upregulated in response to drought treatment for 24h and 48h. Among the highly stress-responsive MtGST members, MtGSTU17 showed strong affinity towards its conventional substrates reduced glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) with the lowest binding energy of-5.7 kcal/mol and -6.5 kcal/mol, respectively. Furthermore, the substrate-binding site residues of MtGSTU17 were found to be highly conserved. These findings will facilitate the further functional and evolutionary characterization of GST genes in Medicago.
Editor: United States: Public Library of Science
Idioma: Inglês

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Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L

Hasan, Md Soyib ; Singh, Vishal ; Islam, Shiful ; Islam, Md Sifatul ; Ahsan, Raju ; Kaundal, Amita ; Islam, Tahmina ; Ghosh, Ajit Chakrabarty, Debasis

United States: Public Library of Science

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