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Physiological characterization of chitin synthase A responsible for the biosynthesis of cuticle chitin in Culex pipiens pallens (Diptera: Culicidae)

Yang, Xiaoshan ; Xu, Yang ; Yin, Qi ; Zhang, Hongbo ; Yin, Haitao ; Sun, Yan ; Ma, Lei ; Zhou, Dan ; Shen, Bo

Parasites & vectors, 2021-05, Vol.14 (1), p.234-234, Article 234 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Physiological characterization of chitin synthase A responsible for the biosynthesis of cuticle chitin in Culex pipiens pallens (Diptera: Culicidae)
Autor: Yang, Xiaoshan ; Xu, Yang ; Yin, Qi ; Zhang, Hongbo ; Yin, Haitao ; Sun, Yan ; Ma, Lei ; Zhou, Dan ; Shen, Bo
Assuntos: Animal cuticle ; Aquatic insects ; Biological research ; Biology, Experimental ; Biosynthesis ; Chitin ; Chitin synthase ; Culex pipiens pallens ; Culicidae ; Cuticles ; Degradation ; Eclosion ; Endoderm ; Enzymes ; Epicuticle ; Gene expression ; Genetic aspects ; Infectious diseases ; Insect ; Insect control ; Insecticide resistance ; Insecticides ; Insects ; Insects as carriers of disease ; Laboratories ; Larvae ; Mesoderm ; Metabolism ; Metamorphosis ; Molting ; Morphogenesis ; Mosquito ; Mosquitoes ; Moulting ; Pathogens ; Pesticide resistance ; Phenotypes ; Physiological aspects ; Physiology ; Pupation ; siRNA ; Structural integrity ; Transferases ; Vertebrates ; West Nile virus
É parte de: Parasites & vectors, 2021-05, Vol.14 (1), p.234-234, Article 234
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: The pathogens transmitted by mosquitoes to humans and animals cause several emerging and resurgent infectious diseases. Increasing insecticide resistance requires rational action to control the target vector population. Chitin is indispensable for insect growth and development and absent from vertebrates and higher plants. Chitin synthase A (CHSA) is a crucial enzyme in chitin synthesis; therefore, identifying and characterizing how CHSA determines chitin content may contribute to the development of novel vector control strategies. The injection of small interfering RNA targeting CHSA (siCHSA) to knockdown CHSA transcripts in larval, pupal and adult stages of Culex pipiens pallens resulted in the appearance of different lethal phenotypes. When larval and pupal stages were injected with siCHSA, CHSA knockdown prevented larval molting, pupation and adult eclosion, and affected the production of chitin and chitin degradation, which resulted in an ecdysis defect phenotype of mosquitoes. When siCHSA was injected into mosquitoes in the adult stage, CHSA knockdown also affected the laminar organization of the mesoderm and the formation of pseudo-orthogonal patterns of the large fibers of the endoderm. We provide a systematic and comprehensive description of the effects of CHSA on morphogenesis and metamorphosis. The results show that CHSA not only affects chitin synthesis during molting, but also might be involved in chitin degradation. Our results further show that CHSA is important for the structural integrity of the adult mosquito cuticle.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Physiological characterization of chitin synthase A responsible for the biosynthesis of cuticle chitin in Culex pipiens pallens (Diptera: Culicidae)

Yang, Xiaoshan ; Xu, Yang ; Yin, Qi ; Zhang, Hongbo ; Yin, Haitao ; Sun, Yan ; Ma, Lei ; Zhou, Dan ; Shen, Bo

England: BioMed Central Ltd

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