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Characteristic Patterns of N Oct-3 Binding to a Set of Neuronal Promoters

Blaud, Magali ; Vossen, Christine ; Joseph, Gérard ; Alazard, Robert ; Erard, Monique ; Nieto, Laurence

Journal of molecular biology, 2004-06, Vol.339 (5), p.1049-1058 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Characteristic Patterns of N Oct-3 Binding to a Set of Neuronal Promoters
Autor: Blaud, Magali ; Vossen, Christine ; Joseph, Gérard ; Alazard, Robert ; Erard, Monique ; Nieto, Laurence
Assuntos: Amino Acid Sequence ; Animals ; Base Sequence ; Circular Dichroism ; Dimerization ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Hepatocyte Nuclear Factor 3-beta ; HNF-3β ; Host Cell Factor C1 ; Humans ; Molecular Sequence Data ; N Oct-3 ; neuronal promoters ; Neurons - physiology ; Nuclear Proteins - metabolism ; Octamer Transcription Factor-1 ; Octamer Transcription Factor-3 ; POU ; Promoter Regions, Genetic ; Protein Binding ; Protein Structure, Secondary ; Protein Subunits - chemistry ; Protein Subunits - metabolism ; Sequence Alignment ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Transcription Factors - metabolism
É parte de: Journal of molecular biology, 2004-06, Vol.339 (5), p.1049-1058
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Descrição: N Oct-3, a neurospecific POU protein, homodimerizes in a non-cooperative fashion on the neuronal aromatic l-amino acid decarboxylase gene promoter and generates heterodimers with HNF-3β. Several other neuronal gene promoters, the corticotropin releasing hormone and the aldolase C gene promoters also contain overlapping binding sites for N Oct-3 and HNF-3β. We have demonstrated that N Oct-3 presents a non-cooperative homodimerization on these two additional targets and can also give rise to heterodimers with HNF-3β. Surprisingly, despite the high degree of conservation of the respective POU subunits, the ubiquitous POU protein Oct-1 can only form monomers even in the presence of either N Oct-3 or HNF-3β on these DNA targets. Our data indicate that this difference is correlated with the specific ability of a portion of the N Oct-3 linker to fold as an α-helix, a property shared by class III POU proteins. These results suggest that this novel binding pattern permits the heterodimerization of N Oct-3 and HNF-3β on the neuronal promoters, which could be a key issue in the development of the nervous system and possibly tumors of neural origin.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Characteristic Patterns of N Oct-3 Binding to a Set of Neuronal Promoters

Blaud, Magali ; Vossen, Christine ; Joseph, Gérard ; Alazard, Robert ; Erard, Monique ; Nieto, Laurence

England: Elsevier Ltd

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